Herbomineral formulations have been used worldwide against various chronic and degenerative diseases, due to its fewer side effects. A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory biomarkers like alteration in immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response of IgM and IgG were altered by 4.54% and 9.49%, respectively in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. On the other hand, the cellular immune response i.e. CD4+ and CD8+ counts were significantly increased by 56.21% and 73.6%, respectively in the G4 group compared with the G2. Hematology analysis showed an increased level of TLC and lymphocytes by 14.85% and 21.42%, respectively in the G4 group compared to the G2. Lipid profile analysis showed a significant decrease in the level of total cholesterol, triglycerides, LDL, and VLDL in the G4 group compared with the G2. Further, results suggests a significant decrease in the hepatic biomarkers such as SGOT, SGPT, and cardiac enzyme, CK-MB by 18.54%, 19.12%, and 26.23%, respectively in G4 group compared to the G2 group. However, the testosterone level was significantly decreased in G4 group and untreated test formulation (G5) groups by 53.87% and 26.25%, respectively compared with the G2 group. Additionally, the antioxidant assay data showed an altered level of tested enzymes such as LPO, SOD and CAT, in the G4 group compared with the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed a significant improved cellular and humoral immunity, hematological and biochemical profile as compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Herbomineral formulations have been used and accepted all around the world owing to its medicinal and therapeutic importance. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) Treatment on the new proprietary herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The Biofield Energy Treated and untreated test formulation was evaluated using experimental parameters such as humoral immune response (primary and secondary antibody titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry were studies. Humoral immune data showed the primary and secondary antibody titre values were significantly (p≤0.05) increased by 105.88% and 260%, respectively in the Biofield Energy Treated Test formulation (G4) group with respect to the disease control group (G2). The delayed type hypersensitivity results showed a significant increased paw edema by 32.43% and 53.33% at 24 and 48 hours, respectively in the Biofield Energy Treated Test formulation (G4) group, while untreated test formulation group (G5) animals showed 13.33% increase in the paw edema (at 48 hour) compared with the G2 group. Hematological analysis showed a significant increase in the red blood cell count, while the platelet count was significantly increased by 27.78% in Biofield Energy Treated Test formulation (G4) group as compared with the G2 group. Biochemical analysis exhibited an increased level of calcium and phosphorus by 1.58% and 12.65%, respectively while serum uric acid was significantly decreased by 36.66% in the G4 group compared with the G2 group. In addition, animal weight parameters like body weight, relative organ weight, histopathology, water and feed intake data suggest that the Biofield Energy Treated herbomineral formulation was found safe, non-toxic, throughout the exposure period. In conclusion, the The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation would be the powerful immunomodulatory product, which can be useful to modulate the immunity against many infectious diseases. Overall, experimental data suggests that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
Skin health and aging are the complex biological process influenced by several intrinsic (or endogenous) and extrinsic (or exogenous) factors. Various skin-based therapies are currently available to rejuvenate the skin, but they might be related with some side-effects such as scarring. The objective of the present study was to evaluate the effect of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the human foreskin fibroblast (HFF-1) cell line and Dulbecco’s Modified Eagle Medium (DMEM) for skin health parameters like cell proliferation and synthesis of collagen. The rate of cellular proliferation in HFF-1 cells was identified, and the results found that the Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased by 152.38% compared to the negative control group. Additionally, the cell proliferation was also significantly increased by 71.43% in the Biofield Energy Treated cells compared to the negative control group. Similarly, the collagen level was significantly (p ≤ 0.001) increased by 60.42% in the Biofield Energy Treated DMEM compared with the negative control group. Hence, the results exhibited a significant improvement of collagen synthesis and cellular proliferation in the Biofield Energy Treated DMEM for improving skin health. It can be concluded that The Trivedi Effect® - Consciousness Energy Healing Treatment might be a complementary and alternative approach with respect to the skin health, anti-aging in DMEM compared with the HFF-1 cell line. Therefore, the Biofield Energy Treated DMEM could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles, etc.
Herbomineral formulations have been used worldwide against various chronic and degenerative diseases due to its fewer side effects. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. Biomarkers like immunoglobulins (IgG, IgM), cluster differentiation (CD4+), CD8+, superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO) were monitored. The level of IgM was increased by 4.76% in the Biofield Energy Treated test formulation (G4) compared to the disease control group (G2). The levels of CD4+ and CD8+ counts were significantly (p≤0.01) increased by 222.22% and 355.36% in the G4 group compared to the G2 group. The level of lymphocyte was increased by 5% and eosinophil count was significantly decreased by 75% in the G4 group compared to the G2 group. The lipid biomarkers such as total cholesterol (TC), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were significantly lowered by 9.70%, 6.67%, and 23.54%, respectively in the G4 group compared to the G2 group. The expression of SOD was reduced by 9.96% in the G4 group compared to the G2 group. Further, LPO expression was significantly reduced by 33.38% and 16.88% in the G4 and untreated test formulation (G5) groups, respectively compared to the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed significantly improved the cellular and humoral immunity, hematological and biochemical profile compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Skin health and aging are co-related with a complex biological process that are influenced by a combination of factors such as intrinsic (or endogenous) and extrinsic (or exogenous). Many skin based therapies including physical and chemical methods are in practice to rejuvenate the skin, but they might be related with some significant side-effects such as scarring. The aim of the present study was to evaluate the effect of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco’s Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level. The cell growth rate was evaluated at 24, 48, and 72 hours and the results observed with a significant growth in the Biofield Treated DMEM group compared with the Biofield Treated HFF-1 cells. The HFF-1 cellular proliferation rate was significantly (p≤0.001) increased by 85% in the Biofield Treated DMEM, while positive controls, ascorbic acid (10µM) and FBS (15% fetal bovine serum) reported with increased cell proliferation by 21% and 44%, respectively. Similarly, the collagen level was significantly (p≤0.01) increased in the Biofield Treated DMEM by 44.2%, while the Biofield Energy Treated cells did not showed significant change in collagen and proliferation rate as compared with control. Hence, the results concluded significant improved collagen and proliferation in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging in DMEM as compared with the HFF-1 cell line.
Cell growth and proliferation plays an important role in wound healing, which is a complex and dynamic process. Wound management and recovery is currently done using various antiseptics and antibiotics in order to prevent or treat infection. The objective of the present study was to assess the effect of Biofield Energy (Consciousness Energy Healing Treatment - The Trivedi Effect®) Treated cell growth medium on the proliferation of human dermal fibroblast cells (HFF-1) for the assessment of skin health promotion potential. An aliquot of HFF-1 cells and DMEM were divided into two parts, one was treated with the Biofield Energy Treatment and denoted as the treated test samples, while other part was coded as the untreated test samples. MTT assay was used to study the cellular proliferation, while cell growth rate was estimated under phase contrast microscope by monitoring the cell growth at 24, 48, and 72 hours interval and photomicrographs were captured. The results of HFF-1 cellular proliferation rate showed a significant (p≤0.001) increased by 80% in the Biofield Energy Treated DMEM, while positive controls, ascorbic acid (10 µM) and FBS (15% fetal bovine serum) showed increased cell proliferation by 21% and 44%, respectively. In addition, cell growth rate was evaluated at 24, 48, and 72 hours and the results were observed with a significant growth in the Biofield Energy Treated DMEM group compared with the Biofield Energy Treated HFF-1 cells. Thus, on the basis of the experimental results, it can be concluded that The Trivedi Effect®-Consciousness Energy Healing Treatment has the significant capacity to improve the cellular proliferation and cell growth rate of human skin cells in the Biofield Energy Treated media (DMEM) for improving the overall skin health. It can be used as a complementary and alternative approach with respect to the various skin-related disorders, anti-aging, wound healing, and many more.
The herbomineral formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in female Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The Biofield Energy Treated group (G3) showed 6.39% increased the CD4+ count compared with the disease control (G2) group. The level of IgM was significantly increased by 23.08% and 46.15% in the G3 group and Biofield Energy Treatment group per se at day -15 (G6), respectively compared with the G2 group. The total leucocyte count (TLC) and neutrophil were significantly increased by 16.15% and 26.69% (p≤0.05), respectively in the G3 group compared with the G2 group. Further, the levels of TLC, eosinophil, and monocyte were significantly increased by 70.57% (p≤0.01), 33.33%, and 8.50%, respectively in the G6 group compared with the G2 group. Low density lipoprotein (LDL) was reduced by 6.24% in the G3 group, while triglyceride (TG) and LDL were reduced by 12.12% and 16.99%, respectively in the G6 group compared with the G2 group. The levels of serum glutamate-pyruvate transaminase (SGPT), alkaline phosphatase (ALP), and total bilirubin were significantly decreased by 20.26%, 32.98%, and 25.00%, respectively in the G3 group compared with the G2 group. Further, SGPT and total bilirubin were reduced by 9.02% and 16.67%, respectively in the G6 group compared with the G2 group. Progesterone was significantly increased by 59.85% in the G3 group, compared with the G2 group. The data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment group per se at day -15 have shown a significant immunomodulatory potential. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data also suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
The use of an herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side-effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal (ashwagandha root extract) and minerals (zinc chloride, magnesium gluconate, and sodium selenate). The objective of this study was to evaluate the immunomodulatory effect of the Biofield Energy Treated test formulation on female Sprague Dawley (SD) rats. The test formulation was divided into two parts, one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Energy Treated formulation. The effect of the test formulation was monitored using humoral immune response, delayed type hypersensitivity, hematology, biochemistry, relative organ weight, and histopathology. The primary hemagglutination (HA) antibody titre level was significantly (p≤0.05) increased by 185.88% in the Biofield Energy Treated group (G3), while 41.18% increased in the untreated test formulation group (G4) as compared to the disease control group (G2). The secondary HA titre was significantly increased by 540% (p≤0.001) and 166.7% in the G3 and G4 groups, respectively as compared to the G2 group. The secondary HA titre was also increased significantly (p≤0.05) by 193% in the Biofield Energy Treated group per se at day -15 (G6), as compared to the G2 group. The paw volume data showed an alteration in the G3 and G6 groups as compared to the G2 group. The platelet count was significantly increased by 11.16% and 8.69% in the G3 and G4 groups, respectively as compared to the G2 group. The magnesium level was increased by 9.31% in the G3 group as compared to the G2 group. The uric acid was significantly reduced by 23.88% in the G3 group, while it was increased by 17.47% in the G4 group as compared to the G2 group. Since, the relative organ weight and histopathological findings did not produce any signs of toxicity as compared to the normal control group (G1). These data suggested that the Biofield Energy Treated herbomineral formulation was found to be safe and showed better immunomodulatory response as compared to the untreated formulation. Thus, it is assumed that the Biofield Energy Treated herbomineral formulation could be better and more useful to boost-up the immune system in healthy human and patients.