Assessment of the Biofield Energy Healing-Based Proprietary Test Formulation for Energy Boosting Biomarker-ATP in MG63 and C2C12 Cell Lines
Health & Wellness
The objective of the present study was to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated Test formulation (TI) containing minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on ATP levels in human osteoblast (MG-63, Osteosarcoma) and mouse myoblast (C2C12) cell lines. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation (UT), while the other portion of the test formulation received Biofield Energy Healing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing Treatment and known as Biofield Energy Treated (BT) test items. MTT results showed that the test formulation in various concentrations was found as safe and nontoxic, with the viability range from 82% to 159%. However, the level of adenosine tri phosphate (ATP) in MG63 cells was increased by 146.9% (at 0.001 μg/mL) in the UT-DMEM+BT-TI group as compared with the UT-DMEM+UTTI group. However, 79.6%, 111.9%, and 180.7% increased the level of ATP was reported at 0.1, 1, and 10 μg/mL, respectively in the BTDMEM+UT-TI group, while 456.9%, 91.5%, and 123.4% increased ATP level was reported at 0.001, 0.01, and 0.1 μg/mL, respectively in the BT-DMEM+BT-TI group as compared with the UT-DMEM+UT-TI group in MG63 cells. Similarly, in the C2C12 cell lines were also reported with significant increased the ATP level by 178.7% at 1 μg/mL in the UT-DMEM+BT-TI group compared to the UTDMEM+UT-TI group. Further, the ATP level was increased by 637.5%, 240.1%, and 26.8% at 0.001, 0.01, and 0.1 μg/mL, respectively in the BT-DMEM+UT-TI group as compared to the UT-DMEM+UT-TI group. Moreover, the ATP level was increased by 776.2%, 22.9%, 234.2%, and 24.5% at 0.001, 0.01, 0.1, and 1 μg/mL, respectively in the BT-DMEM+BT-TI group as compared with the UT-DMEM+UTTI group. Overall, data suggested that there was significant improvement of ATP level in the Biofield Energy Treated test formulation as well as Biofield Treated DMEM medium. Therefore, the results suggest that the Biofield Energy Treated test formulation and/or the Biofield Energy Treated DMEM medium can be used as energy booster and also it can improve the mental stamina, endurance, and physical strength of the muscles to show the better performance.
Trivedi MK, Branton A, Trivedi D, Nayak G and Jana S
<a href="https://www.researchgate.net/publication/376858464_Assessment_of_the_Biofield_Energy_Healing-Based_Proprietary_Test_Formulation_for_Energy_Boosting_Biomarker-ATP_in_MG63_and_C2C12_Cell_Lines">https://www.researchgate.net/publication/376858464_Assessment_of_the_Biofield_Energy_Healing-Based_Proprietary_Test_Formulation_for_Energy_Boosting_Biomarker-ATP_in_MG63_and_C2C12_Cell_Lines</a>
Annex Publishers
March 03, 2020
English
Journal Article
Identification of Endothelin-1 and Nitric Oxide Activity in Aorta, Lung and Kidney after Treatment with the Biofield Energy Treated Proprietary Test Formulation on L-Name and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
Health & Wellness
The study was aimed to investigate the impact of the Trivedi Effect® on high fat diet (HFD) and NG-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced Sprague Dawley rats using various functional organ (aorta, lung, and kidney) biomarkers. The functional biomarkers like endothelin-1 and nitric oxide (NO) were evaluated in all the three tissue homogenate using ELISA. The proprietary test formulation including minerals (selenium, magnesium, zinc, calcium, iron, and copper), vitamins (vitamin B9, ascorbic acid, cyanocobalamin, cholecalciferol, and pyridoxine HCl), cannabidiol isolate, β-carotene, and Panax ginseng extract. The each constituents of the proprietary test formulation were divided into two parts; one was denoted as untreated without any Blessing, while the other part received Biofield Treatment by Mr. Mahendra Kumar Trivedi. The results showed that the level of endothelin-1 in the aorta tissue homogenate was decreased by 52.41%, 50.92%, 42.71%, 32.20%, and 45.66% in the G5, G6, G7, G8, and G9 groups, respectively than disease control (G2) group. The level of NO in the aorta tissue homogenate was increased by 30.77%, 11.02%, 13.96%, and 22.57% in the G5, G6, G7, and G8 than G2. Moreover, the expression of endothelin-1 in the lung tissue homogenate was decreased by 40.95%, 19.69%, 39.94%, 24.09%, and 18.85% in the G5, G6, G7, G8, and G9 groups, respectively than G2. The level of NO in the lung tissue homogenate was increased by 25.29% and 29.16% in the G6 and G7 groups, respectively than G2. Further, the level of endothelin-1 in the kidney tissue homogenate was decreased by 18.65%, 11.27%, 22.84%, 19.44%, and 30.32% in the G5, G6, G7, G8, and G9 groups, respectively than G2. Overall, data suggested a significant improvement of endothelin-1 and nitric oxide activity in different organs. Therefore, the current outcomes could able to envisage a remarkable reduction of cardiovascular complications in the preventive treatment viz. G6, G7, G8, and G9 and might be beneficial various types of cardiovascular disorders and overall health.
Trivedi MK, Branton A, Trivedi D and Jana S
<a href="https://medwinpublishers.com/OAJC/volume.php?volumeId=343&issueId=1085">https://medwinpublishers.com/OAJC/volume.php?volumeId=343&issueId=1085</a>
Medwin Publishers
9-Sep-2021
English
Journal Article
Organ Protective Activities of Biofield Energy Treated Proprietary Test Formulation on Cecal Slurry, LPS and E. Coli Induced Systemic Inflammatory Response Syndrome (SIRS) Model in Sprague Dawley Rats
Health & Wellness
Systemic inflammatory response syndrome (SIRS) is a multiplex pathophysiological defense response against noxious stressor such as infection, trauma, burns, or any others injuries. Study objective was to evaluate the antioxidants and anti-inflammatory potential of Biofield Energy Treated (Blessed) Proprietary Test Formulation and Biofield Energy Healing Treatment (Blessing) per se to the animals on Cecal Slurry, LPS, and E. coli-induced SIRS model in Sprague Dawley rats. Each component of the test formulation was divided into two parts; one part was denoted as untreated test formulation, while other part of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MPO was significantly (p≤0.001) reduced by 51.44%, 71.69%, 55.79%, 55.16%, and 58.12% in G5 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se + Biofield Energy Treated/Blessed test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se animals + untreated test formulation) groups, respectively with reference to disease control (G2) group. The level of LPO end product in terms of malondialdehyde (MDA) was significantly (p≤0.001) reduced by 52.71%, 56.54%, 67.35%, and 75.28% in G6, G7, G8, and G9 as compared to the G4 group. The level of MMP-9 was significantly (p≤0.001) decreased by 34.79%, 48.57%, 39.29%, and 41.25% in G6, G7, G8, and G9, respectively with reference to G4 group. Moreover, the level of FDP was significantly (p≤0.001) decreased by 39.87%, 44.91%, 39.76%, 43.09%, and 46.47% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of substance P was significantly (p≤0.001) decreased by 19.93%, 25.51%, and 27.92% in the G7, G8, and G9 groups, respectively as compared to the G4 group. The level of iNOS was significantly (p≤0.001) decreased by 39.26% (p≤0.001), 38.95% (p≤0.001), 47.63% (p≤0.001), and 59.78% (p≤0.001) in the G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested the antioxidant and anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Consequently, the results significantly slowdown the inflammation-related symptoms in preventive treatment groups like G6, G7, G8, and G9.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
<a href="https://www.scitcentral.com/article.php?journal=62&article=2447">https://www.scitcentral.com/article.php?journal=62&article=2447</a>
SciTech Central
18-Aug-2021
English
Journal Article
Assessment of Biofield Energy Healing-Based Novel Proprietary Test Formulation Comprised of Minerals, Vitamins, Panax ginseng Extract, CBD Isolates, and β-carotene on Senescence of Fibroblasts (WI-38)
Health & Wellness
The study was planned to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated Test formulation (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on senescence of human lung fibroblasts (WI-38) cell line in EMEM medium. Growth rate (amount of doubling in one unit of time) was determined, while senescence was induced by H2O2 damage and then cells were allowed to recover for 48 h. The test formulation constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing Treatment and divided as Biofield Energy Treated (BT) and untreated (UT) test items. MTT data showed that the test formulation in various tested concentrations was found as safe and nontoxic with viability range 70% to 125%. The decreased reduction in doubling time was calculated on the basis of untreated test group of EMEM and test formulation. The UT-EMEM + BT-TI test group reported with decreased doubling time by 67% and 67.5% at 0.001 and 0.1 µg/mL, respectively, while 134.3%, 24.4%, and 50.5% decreased doubling time was found in the BT-EMEM + UT-TI group at 0.001, 0.01, and 0.1 µg/mL respectively, as compared with untreated test group. In addition, BT-EMEM + BT-TI test group showed a significant reduction in doubling time value by 191.6%, 33.2%, and 74.6% at 0.001, 0.01, and 0.1 µg/mL respectively, as compared with the UT-EMEM + UT-TI group. Overall, the results showed the significant decreased doubling time in WI-38 cells. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with significant anti-aging activity and its associated complications/symptoms.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
<a href="https://www.scitcentral.com/article.php?journal=26&article=2448">https://www.scitcentral.com/article.php?journal=26&article=2448</a>
SciTech Central
12-Aug-2021
English
Journal Article
Impact on Fundamental Physiological Functional Biomarkers after Administration of Biofield Treated Formulation in Unpredictable Chronic Stress (UCS)-Induced Rats
Health & Wellness
The aim of the present study was to evaluate the fundamental physiological functional biomarkers analysis of biofield treated/blessed novel test formulation in Unpredictable Chronic Stress (UCS) male Sprague Dawley (SD) rats. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other part of each components and three group of animals received biofield energy healing/blessing treatment by Mr. Mahendra Kumar Trivedi, a famous biofield energy healer. Total Leucocyte Count (TLC) count was significantly (p≤0.001) improved by 75.62%, 64.20%, 44.03%, 82.53%, and 50.83% in the biofield energy treated test formulation to the untreated rats (G5), biofield energy treatment per se to the rats (G6) groups, 15 days pre-treatment of biofield energy treated test formulation (G7), 15 days pre-treatment of biofield energy treated test formulation to the biofield energy treatment per se to rats (G8), and untreated test formulation to the biofield energy treated rats (G9) respectively, as compared with the G2 group. Besides, lymphocytes count were significantly increased by 52.50% (p≤0.001), 47.02% (p≤0.001), 32.21%, 83.86% (p≤0.001), and 40.15% (p≤0.001) in the G5, G6, G7, G8, and G9 groups, correspondingly with reference to G2 group. In addition, monocyte counts were also significantly increased by 82.52% (p≤0.05), 65.06% (p≤0.05), 38.68%, and 17.03% in the G5, G6, G8, and G9 groups, respectively as compared with the G2 group. Biochemical analysis showed that glucose level was maintained in all the experimental test groups, while UCS significantly increased the level of glucose. Biochemical analysis showed that HDL level was significantly (p≤0.001) improved by 37.62%, 46.64%, and 36.27% in the G5, G7, and G9 groups, respectively as compared with the G2. However, total cholesterol was decreased by 14.86%, 30.32%, 44.29%, and 32.66% in the G5, G7, G8, and G9 groups, correspondingly with reference to G2. However, VLDL level was also significantly reduced by 14.91%, 30.70%, 44.30% (p≤0.01), and 32.89% in the G5, G7, G8, and G9 groups, respectively as compared with the G2. Hepatic and cardiac biomarkers analysis showed that ALP level was decreased by 27.97% and 13.37% in the G5 and G9 groups, respectively as compared with the G2. The level of SGOT was significantly (p≤0.01) decreased by 21.45%, 19.19%, and 19.81% in the G5, G7, and G9 groups, respectively as compared with the G2. On the other hand, SGPT level was also altered by 11.32% and 11.72% in the G5 and G6 groups, respectively with reference to G2 group. CK-MB level was significantly reduced by 16.32% and 37.01% (p≤0.01) in the G8 and G9 groups, respectively with reference to G2. Animal weight parameters like body weight, organ to body weight ratio, and feed intake data showed that the changes were non-significant and no toxic effect was observed after the experimental period. Overall, the data suggested a significant improvement of immune-related hematology parameters, CK-MB, SGOT, and lipid profile after biofield treatment and slowdown the disease progression rate and related symptoms in the preventive maintenance groups. Therefore, the data could be benefitted to build-up good body immune responses, enhance resistance towards diseases, and reduce allergies and lethargic conditions.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
<a href="https://crimsonpublishers.com/sbb/fulltext/SBB.000609.php">https://crimsonpublishers.com/sbb/fulltext/SBB.000609.php</a>
Crimson Publishers
18-Jul-2021
English
Journal Article
Oxidative Stress and Inflammatory Biomarkers Analysis of Biofield Treated Proprietary Test Formulation in Heart Tissues in Cecal Slurry, LPS and E. Coli-induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats
Health & Wellness
The study was aimed to evaluate the antioxidant and anti-inflammatory biomarkers in heart tissues after treatment with the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. In this experiment, different antioxidants biomarkers such as myeloperoxidase (MPO), superoxide dismutase (SOD), lipid peroxidase (LPO) and proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), macrophage inflammatory protein-2 (MIP-2), and matrix metallopeptidase 9 (MMP-9) were analysed using ELISA assay in heart homogenate. A proprietary test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and the animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MPO was reduced by 12.07% in the G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15) group as compared to the untreated test formulation (G4) group. Moreover, the level of SOD was significantly (p≤0.05) increased by 19.03%, 17.26%, and 11.81% in the G6, G7, and G9 groups, respectively as compared to the G4 group. The level of TNF-α was significantly decreased by 25.97%, 40.28% (p≤0.01), 24.86%, 36.54% (p≤0.01), and 34.30% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the disease control (G2) group. Moreover, the level of IL-6 was significantly (p≤0.001) decreased by 23.5%, 31.0%, 26.3%, and 39.8% in the G5, G6, G8, and G9 groups, respectively as compared to the G2 group. Additionally, the level of MIP-2 was reduced by 26.7% and 19.5% in the G6 and G8 groups, respectively as compared to the G4 group. Besides, the level of MMP-9 was significantly (p≤0.001) reduced by 15.1%, 21.5%, and 34% in the G6, G8, and G9 groups, respectively as compared to the G4 group. Altogether, the data imply the antioxidant and anti-inflammatory potential of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of cardiac injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
<a href="https://juniperpublishers.com/arr/ARR.MS.ID.555694.php">https://juniperpublishers.com/arr/ARR.MS.ID.555694.php</a>
Juniper Publishers
13-Jul-2021
English
Journal Article
Evaluation of Inflammatory Response after Treatment with the Biofield Energy Treated Proprietary Test Formulation on Combination of Cecal Slurry, LPS and E. coli Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats
Health & Wellness
Sepsis is a systemic inflammatory response to a confirmed or suspected infection. The transition from sepsis to septic shock causes high rate of mortality. The aim of this experiment was to evaluate the anti-inflammatory potential of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats using serum inflammatory biomarkers. In this experiment, various inflammatory biomarkers such as C-reactive protein (CRP), leptin, nitric oxide (NO), substance polypeptide (substance P), matrix metalloproteinase-2 (MMP-2), and monocyte chemoattractant protein-1 (MCP-1) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of CRP was significantly (p≤0.001) reduced by 36.78%, 37.67%, 34.44%, 61.70%, and 43.37% in the G5 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the level of leptin was significantly increased by 11.83% and 16.48% (p≤0.05) in the G5 and G7 groups, respectively as compared to the G2 group. The level of NO was increased by 25.97% in the G6 group as compared to the G2 group. The level of substance P was significantly decreased by 32.62% (p≤0.001), 11.89%, 30.5% (p≤0.001), 11.21%, and 15.64% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Moreover, the level of MMP-2 was significantly decreased by 17.35% (p≤0.05) and 42.98% (p≤0.001) in the G8 and G9 groups, respectively as compared to the G2 group; while 28.43%, 50.62%, and 22.43% decreased in the G6, G8, and G9 groups, respectively as compared to the untreated test formulation group (G4). Additionally, the level of MCP-1 was significantly (p≤0.001) decreased by 53.42%, 53.47%, 55.30%, 54.58%, and 53.88% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
Mahendra Kumar T, Alice B, Dahryn T and Snehasis J
<a href="https://medwinpublishers.com/IJBP/volume.php?volumeId=406&issueId=1237">https://medwinpublishers.com/IJBP/volume.php?volumeId=406&issueId=1237</a>
Medwin Publishers
13-Aug-2021
English
Journal Article
Neuroprotective (iNOS), fibrinolytic (FDP) Markers Analysis of Biofield Treated Proprietary Test Formulation on Cecal Slurry, LPS and E. coli Induced Systemic Inflammatory Response Syndrome (SIRS) Model in Sprague Dawley Rats
Health & Wellness
The aim of this experiment was to evaluate the antioxidants and anti-inflammatory potential of the Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS, and E. coliinduced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. The parameters like in brain (MMP-9, FDP, Substance P, iNOS) and in heart (FDP, substance P, iNOS) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MMP-9 in brain was significantly (p≤0.001) reduced by 48.63%, 36.81%, 40.84%, and 60.03% in the G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the untreated test formulation (G4) group. The level of FDP in brain was significantly reduced by 43.10% (p≤0.001), 33.40%, 35.46%, and 62.11% (p≤0.001) in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. The level of substance P was significantly decreased in brain by 18.42% (p≤0.001), 53.79% (p≤0.001), 16.65%, 14.64%, and 28.43% (p≤0.001) in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G4 group. The iNOS level in brain was significantly decreased by 35.92% (p≤0.001), 32.80%, 47.20% (p≤0.001), and 66.43% (p≤0.001) in the G6, G7, G8, and G9 groups, respectively than G4 group. Besides, FDP in heart tissue was significantly decreased by 15.66%, 17.41% (p≤0.05), 11.85%, and 13.02% in the G5, G6, G8, and G9 groups, respectively as compared to the G4 group. The levels of heart substance P and iNOS were altered. Overall, the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive maintenance groups showed an antioxidant and anti-inflammatory potentials. Therefore, the results showed the significant slowdown the inflammationrelated disease progression and its complications/symptoms in the preventive groups (viz. G6, G7, G8, and G9) that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries.
Mahendra Kumar T, Alice B, Dahryn T and Snehasis J
<a href="https://medwinpublishers.com/JIDTM/volume.php?volumeId=382&issueId=1236">https://medwinpublishers.com/JIDTM/volume.php?volumeId=382&issueId=1236</a>
Medwin Publishers
3-Aug-2021
English
Journal Article
Estimation of Mechanistic Bio markers in Multiple Organs after Administration of Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
Health & Wellness
Cardiovascular disorders (CVDs) are one of the most leading cause of death worldwide. Study objective was to evaluate the impact of the Biofield Energy Treated/Blessed novel Proprietary Test Formulation and Biofield Energy Healing Treatment/Blessing per se to the animals on NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) and high fat diet (HFD)-induced cardiovascular model in Sprague Dawley rats using various functional organ (liver, heart, kidney, brain and muscles) biomarkers. The functional biomarkers like vitamin D receptor (VDR), telomerase enzyme, and citrate synthase using ELISA assay for the assessment of overall health benefits. Each constituent of the test formulation was divided into two parts; one part was known as the untreated test formulation. The other part of each ingredient and of animals (three group) were received Biofield Energy Healing Treatment/Blessing remotely for -3 minutes by Mr. Mahendra Kumar Trivedi, a renowned spiritual leader. The result showed that the level of VDR expression in the liver tissue was significantly increased by 25.12% and 30.03% in the GS (L-NAME + HFD + the Biofield Energy Treated test formulation) and G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15) groups, respectively, as compared to the (L-N AME+ HFD + untreated test formulation) group (G4). Moreover, the level of VDR in the kidney tissue was also increased by 21.08%, 31.30%, 15.12%, 17.12%, and 15.06% in the GS, G6, G7 (L-NAME + HFD + the Biofield Treated/Blessed test formulation from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment/Blessingperse plus the Biofield Energy Treated/Blessed test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Blessed animals with untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the expression of VDR in the heart tissue was significantly increased by 25.23%, 25.45%, and 19.42% in the GS, G6, and G8 groups, respectively as compared to the G2 group. Further, the level of telomerase enzyme in the brain tissue was increased by 88.67%, 78.59%, 120.29%, 93.24%, and 87.63% in the GS, G6, G7, G8, and G9 groups, respectively than G2 group. Further, the level of citrate synthase was increased marginally in the treatment groups as compared to the disease control and untreated test formulation group. Overall, data suggested a significant improvement ofVDR expression and telomerase enzyme activity in different tissue homogenate, Thus, the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animals that might be beneficial various types of cardiovascular disorders and overall health. Therefore, the study outcomes showed the significant slowdown the oxidative stress-induced cardiovascular disorders and its related symptoms in the preventive treatment groups (viz. G6, G7, G8, and G9) compared to disease control group.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
<a href="https://www.scitcentral.com/article.php?journal=43&article=2431">https://www.scitcentral.com/article.php?journal=43&article=2431</a>
SciTech Central
19-Jul-2021
English
Journal Article
Evaluation of Anti-inflammatory Activity after Treatment with the Biofield Energy Treated Proprietary Test Formulation on Combination of Cecal Slurry, LPS and E. Coli Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats
Health & Wellness
The aim of this study was to evaluate the anti-inflammatory response of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats using serum inflammatory biomarkers. In this experiment, various inflammatory biomarkers such as monokine induced by gamma interferon (MIG), regulated on activation, normal T cell expressed and secreted (RANTES), macrophage inflammatory protein-2 (MIP-2), matrix metallopeptidase 9 (MMP-9), troponin-1, procalcitonin, fibrinogen degradation product (FDP) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of MIG was significantly (p≤0.01) reduced by 48.57%, 56.14%, 47.95%, 51.92%, and 47.83% in the G5 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the level of regulated on activation, normal T cell expressed and secreted (RANTES) was significantly (p≤0.001) decreased by 47.35%, 46.32%, 40.73%, 50.47%, and 39.96% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of macrophage inflammatory protein-2 (MIP-2) was significantly (p≤0.001) decreased by 91.74%, 93.54%, 92.70%, 94.46%, and 93.80% in the G5, G6, G7, G8, and G9 groups, as compared to the G2 group. The level of MMP-9 was significantly (p≤0.001) decreased by 18.74%, 35.54%, 26.48%, 38.16%, and 22.52% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Moreover, the level of troponin-1 was decreased by 28.53%, 28.50%, 14.14%, 30.68%, and 36.34% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the untreated test formulation (G4) group. Additionally, the level of procalcitonin was significantly (p≤0.001) decreased by 27.27%, 51.73%, 46.40%, 54.65%, and 39.08% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Further, the level of fibrinogen degradation product (FDP) was significantly (p≤0.001) decreased by 66.7%, 62.5%, 31.5%, 51.6% and 40.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
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Juniper Publishers
30-Jul-2021
English
Journal Article