Anti‑inflammatory potential of cannabidiol (CBD) on combination of caecal slurry, LPS, and E. coli‑induced systemic inflammatory response syndrome (SIRS) in Sprague Dawley Rats
Health & Wellness
Objective
The study objective was to evaluate the therapeutic effect of cannabidiol (CBD) on a combination of caecal slurry, lipopolysaccharide (LPS), and Escherichia coli (E. coli)-induced systemic inflammatory response syndrome (SIRS) in male Sprague Dawley rats.
Methods
The therapeutic activity was monitored in behavioral tests and inflammatory biomarkers by the enzyme-linked immune sorbent assay (ELISA) method.
Results
Behavioral tasks were significantly increased like a tail flick response by 73.84% (p ≤ 0.001), grip strength by 33.56% (p ≤ 0.028), locomotor activity by 20.71% (p = 0.034) in the CBD (60 mg/kg) group compared to disease control (DC) group. Levels of inflammatory serum biomarkers like interleukin-1β (IL-1β), matrix metallopeptidase-9 (MMP-9), IL-6, and tumor necrosis factor-alpha (TNF-α) were significantly decreased by 29.56 (p = 0.041), 71.20 (p ≤ 0.001), 35.05 (p ≤ 0.001), and 75.56% (p = 0.002), respectively, in the CBD-60 compared with DC. Inflammatory cytokines levels, viz. IL-1β, MMP-9, IL-6, and TNF-α, in the liver were significantly (p ≤ 0.001) decreased by 81.01, 40.41, 22.84, and 69.46%, respectively, in CBD-60 to DC. Similarly, levels of inflammatory cytokines such as IL-1β and MMP-9 in the kidney were significantly (p ≤ 0.001) decreased by 80.90 and 43.93%, respectively, in CBD-60 compared to DC.
Conclusion
Taken together, results suggest that CBD treatment significantly improved behavioral tasks and decreased the level of inflammatory cytokines under SIRS conditions that might provide an opportunity to manage acute and chronic inflammatory disorders.
Mahendra Kumar Trivedi, Sambhu Mondal, Mayank Gangwar, Snehasis Jana
<a href="https://link.springer.com/article/10.1007/s10787-021-00901-z">https://link.springer.com/article/10.1007/s10787-021-00901-z</a>
Springer
8-Jan-2022
English
Journal Article
Effect on the Expression of Serum Cytokines after Administration of Biofield Treated Proprietary Test Formulation in TNBS-induced Ulcerative Colitis Rats
Health & Wellness
The study was aimed to evaluate the gut health protective effect of Biofield Treated/Blessed proprietary test formulation on serum cytokines. Each constituents of the novel test formulation was divided into two parts, one part was denoted as control and the other part was defined as Biofield Energy Treated/Blessed test formulation, which received Biofield Energy Healing Treatment/Blessing (prayer) remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The serum was subjected to test the following biomarkers viz. C-reactive protein (CRP), myeloperoxidase (MPO), and pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), IL-10, IL-23, interferon gamma (IFN-γ), and transforming growth factor beta (TGF-β) using ELISA method. CRP level was decreased by 17.12%, 40.13%, 34.79%, 42.28%, and 12.88% in the G5 (Biofield Treated test formulation), G6 (Biofield Energy Treatment per se at day -15), G7 (Biofield Treated test formulation from day-15), G8 (Biofield Treatment/Blessing per se to the animals with Biofield Treated/Blessed test formulation from day -15), and G9 (Biofield Treatment/Blessing per se from day -15 to animals plus untreated test formulation) groups, respectively as compared with the disease control (G2) group. TGF-β level was decreased by 9.51% and 9.99% in the G7 and G8 groups, respectively than untreated test formulation (G4) group. In addition, the level of IL-23 was decreased by 22.86%, 30.61%, 25.69%, 42.15%, and 25.81% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Further, the level of IL-6 was significantly decreased by 54.4% (p≤0.05), 30.5%, 20%, and 48% (p≤0.05) in the G5, G6, G7, and G8 groups, respectively with respect to the G4 group. There was an alteration of TNF-α compared to G2. Besides, IL-10 level was increased by 138.63%, 169.26%, 106.07%, 102.79%, and 81.55% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. However, the serum MPO level was significantly decreased by 21.33% (p≤0.05), 15.42%, 22.61% (p≤0.05), 13.21%, and 26.25% (p≤0.05) in G5, G6, G7, G8, and G9 respectively, than G2 group. The Trivedi Effect®-Consciousness Energy Healing/Blessing (prayer) would be the best alternative approach for treating gut inflammatory disorders. Thus, the results showed the significant retard the inflammation-related to gastrointestinal diseases (Crohn’s disease, ulcerative colitis, along with microbial, parasitic, and viral inflammatory disorders), and its complications in the preventive treatment groups (viz. G6, G7, G8, and G9).
Mahendra Kumar Trivedi and Snehasis Jana
Juniper Publishers
15-Jun-2021
English
Journal Article
Evaluation of Stress Biomakers (Cytokines) in Presence of Unpredictable Chronic Stress (UCS) in Sprague Dawley Rats after Treatment with the Biofield Energy Treated Novel Proprietary Test Formulation
Health & Wellness
Unpredictable chronic stress (UCS) model was used to evaluate the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats for the estimation of serum cytokines such as TNF-Alpha, IFN-Gamma, IL-6, and IL-2, along with C-reactive protein (CRP) using ELISA assay. A test formulation was formulated including minerals (calcium, copper, iron, magnesium, selenium, and zinc), vitamins (ascorbic acid, alpha tocopherol, cholecalciferol, cyanocobalamin, and pyridoxine HCl), cannabidiol isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one part of each ingredient was defined as the untreated test formulation, while the other parts and three groups of animal received Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The cytokines estimation results showed that the level of IL-2 was reduced by 14.2% in Biofield Energy Treatment per se to the rats (G6) group as compared with the disease control group (G2). TNF-α level in serum was decreased by 11% in the G6 group as compared with the G2 group. IL-6 level in serum was decreased by 21.7%, 30.8%, 16.3%, 10.1%, and 13.4% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. However, IFN-γ level was decreased by 18.3% and 11% in the G6 and G9 groups, respectively as compared with the G4. Similarly, CRP level was significantly decreased by 25.8%, 15.2%, and 14.6% in the G5, G6, and G7 groups respectively, as compared with the G4. All-inclusive, Mr. Trivedi’s Biofield Energy Healing Treatment/Blessing per se along with other preventive treatment groups showed a significant impact on various inflammatory disorders (gout, inflammatory arthritis myositis, rheumatoid arthritis, scleroderma, etc.). Therefore, the outcomes could be slowdown the stress-related disease progression and its complications/symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).
Trivedi MK, Branton A, Trivedi D and Jana S
<a href="https://medwinpublishers.com/AABSc/volume.php?volumeId=367&issueId=1211">https://medwinpublishers.com/AABSc/volume.php?volumeId=367&issueId=1211</a>
Medwin Publishers
7-Jul-2021
English
Journal Article
Evaluation of Inflammatory Serum Biomarkers after Treatment with the Consciousness Energy Healing-Based Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
Health & Wellness
The aim of this experiment was to evaluate the anti-inflammatory potential of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats using serum biomarkers. In this experiment various proinflammatory cytokines such as TNF-α, IL-6, IL-18, and IL-1β, along with C - reactive protein (CRP) using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one part was denoted as the untreated, while the other part and three groups of animals received Biofield Energy Healing Treatment/Blessing remotely for about 3 minutes by a renowned Spiritual Energy Healer, Mr. Mahendra Kumar Trivedi. The cytokines estimation results showed that the level of tumour necrosis factor-alpha (TNF-α) was reduced by 12.72% in the G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15) group as compared to the disease control (L-NAME + HFD + 0.5% CMC) group (G2). Moreover, the level of interleukin-6 (IL-6) was reduced by 31.75%, 15.96%, 24.18%, and 24.11% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the G2 group. Additionally, the level of interleukin-18 (IL-18) was decreased minimally in the treatment group as compared to the G2 group. The level of IL-1β was decreased by 7.93%, 8.35%, 7.93%, 6.68%, and 6.68% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the untreated test formulation (G4). Besides, the level of CRP was decreased by 33.25%, 25.78%, 15.63%, 42.12%, and 35.40% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance antiinflammatory potentials of the Biofield Energy Treated/Blessed test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its complications in the preventive treatment groups (viz. G6, G7, G8, and G9).
Trivedi MK, Branton A, Trivedi D and Jana S
<a href="https://medwinpublishers.com/CCLSJ/volume.php?volumeId=391&issueId=1136">https://medwinpublishers.com/CCLSJ/volume.php?volumeId=391&issueId=1136</a>
Medwin Publishers
23-Jun-2021
English
Journal Article
Effect of Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Biofield Treated Mouse Splenocyte Cells: Impact of the Trivedi Effect®
Nutraceuticals
<p style="text-align:justify;">A proprietary herbomineral formulation was formulated with four ingredients, a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in splenocyte cells, which were isolated from Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. The splenocyte cells were treated with the test formulation at concentrations ranges from 0.00001053 to 10.53 µg/mL and analyzed after 48 hours of treatment by MTT assay. The cell viability data showed safe concentrations up to 1.053 µg/mL with viability ranges from 69.22% to 123.88% in the test formulation groups. The expression of TNF-α was decreased by 4.82% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 2.02%, 4.92%, and 18.78% at 0.00001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation group as compared to the untreated test formulation. The expression of IL-1β was significantly reduced by 83.65%, 92.15%, 27.30%, and 41.88% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. The Biofield Treated test formulation showed significant reduction of IL-1β by 17.26%, 92.61% (p≤0.001), 34.62% (p≤0.05), and 16.13% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Additionally, the expression of chemokine MIP-1α was significantly reduced by 17.03%, 10.99%, 22.33%, 24.21%, 21.61%, and 30.67% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared with the vehicle control. The MIP-1α expression was significantly reduced by 19.32% and 12.56% at 0.01053 and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The overall results demonstrated that the Biofield Energy Treated test formulation significantly down-regulated the expression of TNF-α, IL-1β, and MIP-1α in the Biofield Treated mice splenocyte cells compared to the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Olga Mirgalijeva, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20160406.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20160406.11</a>
Science Publishing Group
December 20, 2016
English
Journal Article
10.11648/j.ajbio.20160406.11
Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells
Nutraceuticals
<p style="text-align:justify;">The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Sambhu Charan Mondal, Snehasis Jana,
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20160406.15" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20160406.15</a>
Science Publishing Group
December 08, 2016
English
Journal Article
10.11648/j.ajhr.20160406.15