Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.
The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.