In Vitro Evaluation of Biofield Treatment on Cancer Biomarkers Involved in Endometrial and Prostate Cancer Cell Lines
Cancer
<p style="text-align:justify;">Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Trivedi’s biofield treatment, referred as treated. Control and treated samples were studied for the determination of cancer biomarkers such as multifunctional cytokines viz. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), interleukin-2 receptor (IL-2R), prostate specific antigen (PSA), and free prostate specific antigen (FPSA) concentrations using ELISA assay on day 10. Experimental results showed a significant reduction of IL-6 level in endometrium (12%) and prostate (98.8%) cancer cell lines while a significant increase was observed in TNF-α level in endometrium (385%) and prostate (89.8%) cancer cell lines as compared to control. No alteration of PSA level was observed in biofield treated endometrium and prostate cell line. Similarly, no alterations were evident in IL-2R and FPSA levels in endometrium and prostate cell lines after biofield treatment as compared to control. In conclusion, results suggest that biofield treatment has shown significant alterations in the level of cytokines (IL-6 and TNF-α) in both endometrium and prostate cancer cell lines.</p>
Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/in-vitro-evaluation-of-biofield-treatment-on-cancer-biomarkers-involved-in-endometrial-and-prostate-cancer-cell-lines-1948-5956-1000358.php?aid=58658" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/in-vitro-evaluation-of-biofield-treatment-on-cancer-biomarkers-involved-in-endometrial-and-prostate-cancer-cell-lines-1948-5956-1000358.php?aid=58658</a>
Omics Publishing Group
August 03, 2015
English
Journal Article
10.4172/1948-5956.1000358
Effect of Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Biofield Treated Mouse Splenocyte Cells: Impact of the Trivedi Effect®
Nutraceuticals
<p style="text-align:justify;">A proprietary herbomineral formulation was formulated with four ingredients, a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in splenocyte cells, which were isolated from Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. The splenocyte cells were treated with the test formulation at concentrations ranges from 0.00001053 to 10.53 µg/mL and analyzed after 48 hours of treatment by MTT assay. The cell viability data showed safe concentrations up to 1.053 µg/mL with viability ranges from 69.22% to 123.88% in the test formulation groups. The expression of TNF-α was decreased by 4.82% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 2.02%, 4.92%, and 18.78% at 0.00001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation group as compared to the untreated test formulation. The expression of IL-1β was significantly reduced by 83.65%, 92.15%, 27.30%, and 41.88% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. The Biofield Treated test formulation showed significant reduction of IL-1β by 17.26%, 92.61% (p≤0.001), 34.62% (p≤0.05), and 16.13% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Additionally, the expression of chemokine MIP-1α was significantly reduced by 17.03%, 10.99%, 22.33%, 24.21%, 21.61%, and 30.67% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared with the vehicle control. The MIP-1α expression was significantly reduced by 19.32% and 12.56% at 0.01053 and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The overall results demonstrated that the Biofield Energy Treated test formulation significantly down-regulated the expression of TNF-α, IL-1β, and MIP-1α in the Biofield Treated mice splenocyte cells compared to the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Olga Mirgalijeva, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20160406.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20160406.11</a>
Science Publishing Group
December 20, 2016
English
Journal Article
10.11648/j.ajbio.20160406.11
Effect of the Consciousness Energy Healing Treatment on DMEM for the Proliferation and Differentiation of Human Bone Osteosarcoma Cells to Improve Bone Health
Bone health
<p style="text-align:justify;">The bone health is an important part of healthy-life and longevity in current situation due to huge toxins and contaminants in the environment and food chain. Considering the importance of bone health in the modern era, the present study was undertaken to investigate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment on Dulbecco's Modified Eagle Medium (DMEM) in which the human bone osteosarcoma cells - MG-63 (ATCC® CRL-1427™) was grown for the assessment of bone cell proliferation and differentiation in vitro. The study parameters were assessed using cell viability by MTT assay, alkaline phosphatase (ALP), and collagen synthesis on bone health using ELISA-based assay. The cell viability was significantly increased by 24% in the Biofield Energy Treated group supplemented with 10% charcoal-dextran with fetal bovine serum (CD-FBS) (G3) compared to the untreated cells group (G1). The level of ALP was significantly increased by 72% in the G3 group compared to the G1 group. Additionally, the level of collagen synthesis was significantly (p≤0.001) increased by 19% in the G3 group compared to the G1 group. The overall results demonstrated that the Biofield Energy Treated DMEM has the potential for bone mineralization and bone cells growth as evident via increased levels of collagen and ALP. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment could be useful as a bone health promoter for various bone-related disorders like low bone density, osteogenesis imperfecta, and osteoporosis, etc.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.tjsr.org/journal/index.php/tjsr/article/view/72">http://www.tjsr.org/journal/index.php/tjsr/article/view/72</a>
Trend Research Publishing
October 10, 2018
English
Journal Article
10.31586/Biochemistry.0303.04
Effect of Biofield Energy Treatment on Bone Cell Proliferation and Differentiation for the Assessment of its Potential to Improve Bone Health
Bone health
<p style="text-align:justify;">The bone health is an important part of healthy-life and longevity in general. The present study was investigated to see the effect of the Biofield Energy Healing (The Trivedi Effect®) on the human bone osteosarcoma cells - MG-63 (ATCC® CRL-1427™) for the assessment of bone cell proliferation and differentiation in vitro. The study parameters were assessed using cell viability by MTT, collagen synthesis, and alkaline phosphatase (ALP) on bone health using ELISA-based assay. The cell viability assay data showed significant response in all the tested groups; while in the Biofield Energy Treated group supplemented with 10% charcoal-dextran treated fetal bovine serum (CD-FBS) (G3) showed better response (increased 63%) in terms of cells proliferation compared to the untreated cell group (G1). The level of ALP was increased by 32% in the G3 group compared to the untreated cells group (G1). Additionally, the level of collagen synthesis was increased by 27% in the G3 group compared to the G1 group. The overall results demonstrated that the Biofield Energy Treatment has the potential for bone mineralization and bone growth as evident via increased levels of collagen and ALP. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a bone health promoter for various bone-related disorders like low bone density, osteogenesis imperfecta, and osteoporosis.</p>
Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis Jana
Medwin Publishers
November 01, 2018
English
Journal Article
10.23880/jobd-16000166
Immunomodulatory Effect of the Biofield Energy Treated Cell Growth Medium (DMEM) and FBS in Immune Cells (Murine Macrophage RAW 264.7)
Immunity
<p style="text-align:justify;">The objective of the present study was to evaluate the immunomodulatory effect of Biofield Energy Treatment (The Trivedi Effect®) on Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) in murine macrophage cells (RAW 264.7). The study parameters were evaluated using cell viability by MTT assay and estimation of proinflammatory cytokine like tumor necrosis factor - alpha (TNF-α) on immunomodulation using enzyme linked immune sorbent assay (ELISA). The cell viability using MTT assay data showed that more than 80% cell viability was observed in all the tested groups compared to the baseline control group (G1). The characteristics of cell morphology in the Biofield Energy Treated DMEM without FBS (G6) group showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation compared to the G1 group. The level of TNF-α expression was significantly reduced by 23.15% in the G3 group compared to the G1 group. Moreover, the secretion of TNF-α was altered by 38.06%, 35.77%, and 282.49% in the G4, G5, and G6 groups, respectively compared to the G1 group. The overall results demonstrated that the Trivedi Effect® - Consciousness Energy Healing Treatment has an impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF-α expression in immune cell (RAW 264.7). Therefore, the Trivedi Effect® treated DMEM and FBS might be useful as an immunomodulator for various immune-related disorders like Graves’ disease, rheumatoid arthritis, multiple sclerosis, etc.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="https://www.ommegaonline.org/article-details/Immunomodulatory-Effect-of-the-Biofield-Energy-Treated-Cell-Growth-Medium-DMEM-and-FBS-in-Immune-Cells-Murine-Macrophage-RAW-2647/1968">https://www.ommegaonline.org/article-details/Immunomodulatory-Effect-of-the-Biofield-Energy-Treated-Cell-Growth-Medium-DMEM-and-FBS-in-Immune-Cells-Murine-Macrophage-RAW-2647/1968</a>
Ommega Publishers
December 27, 2018
English
Journal Article
10.15436/2471-5891.18.1946
Effect of the Biofield Energy Treated Proprietary Test Formulation for Sleep Biomarkers in the Unpredictable Chronic Stress (UCS) Animal Model
Health & Wellness
Sleep biomarkers in brain such as melatonin, BDNF (Brain-derived neurotrophic factor), PGD2 (Prostaglandin D2), leptin, orexin-A, and acetylcholine were evaluated in the unpredictable chronic stress (UCS) rodent model in the presence of Consciousness Energy Healing Treated (the Trivedi Effect®) novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. The test formulation was consisted of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), β-carotene, ginseng, and cannabidiol isolate. The test formulation constituents were divided into two parts, one part of each ingredient was distinct as the untreated test formulation, while the other portion of the test formulation and a group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of melatonin in groups viz. G5 (Biofield Energy Treated Test formulation) and G7 (15-days pre-treatment of Biofield Energy Treated Test formulation) was significantly increased by 17.6% (p≤0.01) and 16%, respectively as compared with the disease control group (G2). Brain-derived neurotrophic factor (BDNF) level in brain was increased by 5.2% in G7 group as compared with the G4. Prostaglandins D2 (PGD2) level was significantly (p≤0.001) increased by 12.7%, 18.1%, 23.7%, and 30.7% in the G6, G7, G8 (15 days pre-treatment of Biofield Energy Treated Test formulation to the Biofield Energy Treatment per se rats), and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups, respectively as compared with the G2. The level of leptin after Biofield Energy Treatment and with the test formulation was altered. However, orexin-A level was significantly decreased by 37.1% (p≤0.05), 32.6%, 40.5% (p≤0.05), 44.4% (p≤0.05), and 28.2% in the G5, G6, G7, G8, and G9 groups respectively, as compared with the G2. Similarly, acetylcholine (Ach) level was significantly (p≤0.001) decreased by 42.5%, 49.2%, 40.1%, 47.9%, and 45% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive in the Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group. The Trivedi Effect® showed increased level of melatonin and decreased levels of insomnia related brain biomarkers which might be helpful to induce better sleep in human.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis Jana
<a href="https://openaccesspub.org/jbtm/article/1253">https://openaccesspub.org/jbtm/article/1253</a>
Open Access Pub
18/Jan/2020
English
Journal Article
Investigation of Biofield Energy Treated Proprietary Test Formulation for Cognition Biomarkers in Brain and CSF Using Unpredictable Chronic Stress (UCS) Animal Model
Health & Wellness
Cognition biomarkers in brain (GABA, Glutamate, Beta Endorphin) and CSF (Corticosterone, KLOTHO, and Serotonin) were evaluated using unpredictable chronic stress (UCS) rodent model in presence of Consciousness Energy Healing Treated (known as the Trivedi Effect®) novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. The test formulation was consisted of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), β-carotene, ginseng, and cannabidiol isolate. The test formulation constituents were divided into two parts, one part of each ingredient was distinct as the untreated test formulation, while the other portion of the test formulation and a group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of brain GABA in groups viz. G7 (15-days pre-treatment of the Biofield Energy Treated Test formulation) and G8 (15-days pre-treatment of the Biofield Energy Treated Test formulation to the Biofield Energy Treatment per se rats) was increased by 12.6% and 14.3%, respectively as compared with the disease control group (G2). Brain glutamate was increased by 20.6%, 36.5%, 25.3%, and 40.7% in the G6 (Biofield Energy Treatment per se to the rats), G7, G8, and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups respectively, as compared with the G2. Brain beta endorphin was significantly increased by 114.3% and 112% in the G5 (Biofield Energy Treated Test formulation to the untreated rats) and G6 groups respectively, as compared with the G4. Similarly, the level of corticosterone in CSF was significantly decreased by 25%, 28.7%, 25%, 27.7%, and 26.9% in the G5, G6, G7, G8, and G9 groups respectively, as compared with the G2. The level of KLOTHO in CSF was significantly increased by 17.6%, 83.3%, 36.4%, and 102.8% in the G5, G6, G7, and G8 groups respectively, as compared with the G4. These data suggested that the Biofield Energy per se significantly reduce the stress-related biomarker such as corticosterone and significantly improve cognition related biomarkers such as GABA, Glutamate, Beta Endorphin, and KLOTHO protein. The brain neurotransmitters (GABA, and glutamate) level was significantly increased in the different treatment groups as compared to the disease control group, which played a vital role in animal’s cognitive functions. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in rats in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group.
Trivedi MK, Branton A, Trivedi D, Nayak G and Jana S
Academic Strive
16/Jan/2020
English
Journal Article
Antioxidant and Anti-inflammatory Activities of the Biofield Energy Healing Based Proprietary Test Formulation in Unpredictable Chronic Stress (UCS) Induced Sprague Dawley Rats Model
Health & Wellness
The oxidative stress plays an important role in the pathogenesis of anxiety and depression. Antioxidant assay was performed in brain tissue to evaluate the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats along with pro-inflammatory cytokines estimation using ELISA assay. The test formulation included the combination of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. All the constituents of the test formulation were divided into two parts; one portion was defined as the untreated test formulation, while the other portion of the test formulation and the animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Antioxidant assay data showed that superoxide dismutase (SOD) level was increased by 50.9%, 18.4%, 47.6%, 24%, and 27.4% in the G5 group, which was received Biofield Energy Treated Test formulation, Biofield Energy Treatment per se to the rats (G6), 15-days pre-treatment of the Biofield Energy Treated test formulation (G7), 15-days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se to rats (G8), and untreated test formulation to the Biofield Energy Treated rats (G9) groups, respectively as compared with the disease control group (G2). The level of lipid peroxidation (LPO) in the brain was significantly decreased by 20%, 21.5%, and 10% in the G5, G6, and G9 groups, respectively as compared with the disease control group (G2). However, IL-6 level in the brain was significantly decreased by 35.9%, 18.2%, 10.5%, 26.9%, and 32.6% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. The TNF-α level in brain was decreased by 73.3%, 57.2%, 63%, 80.5%, and 72.7% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. The IL-1ß level in brain was decreased by 40.2%, 37.8%, 26.7%, 43.5%, and 49.9% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Overall, the data suggested that there was significant antioxidant and anti-inflammatory effect observed for the Biofield Energy per se along with preventive maintenance groups in the preclinical rat model study. Therefore, the results showed the significant slowdown the stress related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group (G2). This energy treatment (the Trivedi Effect®) might also be beneficial against various oxidative stress-related disorders (Parkinson’s disease, Alzheimer’s disease, diabetes, and cardiovascular conditions such as high blood pressure, atherosclerosis, and stroke, etc.) and inflammatory disorders (asthma, autoimmune diseases, coeliac disease, glomerulonephritis, hepatitis, inflammatory bowel disease, etc.).
"Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak and
Snehasis Jana"
<a href="https://crimsonpublishers.com/acam/fulltext/ACAM.000624.php">https://crimsonpublishers.com/acam/fulltext/ACAM.000624.php</a>
Crimson Publishers
14-Feb-2020
English
Journal Article
Evaluation of Vitamin D Receptor (VDR) Expression and Telomerase Activity after Treatment with the Biofield Energy Treated Proprietary Test Formulation in Unpredictable Chronic Stress (UCS)-Induced Sprague Dawley Rats
Health & Wellness
Vitamin D receptor (VDR) expression and telomerase enzyme activity was performed in the unpredictable chronic stress (UCS) model for the evaluation of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. A unique test formulation of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), and nutraceuticals (β-carotene, ginseng, and cannabidiol isolate) was formulated. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals were received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. VDR in liver showed an increased expression in the Biofield Energy Treated Test formulation to the untreated rats (G5), G7 (15-days pre-treatment of Biofield Energy Treated Test formulation), and G8 (15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se rats) groups by 16.7%, 75.7%, and 14.3%, respectively as compared with the untreated test formulation group (G4). Similarly, the VDR expression in kidney was increased by 22.3% and 13.6% in the G6 (Biofield Energy Treatment per se to the rats) and G8 groups, respectively, as compared with the G4. However, the VDR expression in heart was altered by 20.2%, 26%, and 28.2% in the G5, G7, and G8 groups respectively, as compared with the G4. The telomerase activity in the brain was significantly increased by 16.9%, 116%, 258.6% (p≤0.01), 148.1%, and 54.7% in the G5, G6, G7, G8, and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups respectively, as compared with the G4. Similarly, telomerase activity in heart was also increased by 11.1% in the G8 group as compared with the G4. Overall, the experimental data suggested significance effect of Biofield Energy per se along with preventive measure on the animal with respect to various stress-related disorders. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control and untreated test formulation groups.
Trivedi MK and Jana S
<a href="https://medwinpublishers.com/CCLSJ/volume.php?volumeId=391&issueId=1136">https://medwinpublishers.com/CCLSJ/volume.php?volumeId=391&issueId=1136</a>
Medwin Publishers
16-Apr-2021
English
Journal Article
Evaluation of Multiple Vital Serum Biomarkers after Treatment with the Consciousness Energy Healing Based Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
Health & Wellness
The experiment was aimed to evaluate the effect of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on different vital functional serum biomarkers to the animals on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the functional serum biomarkers such as epinephrine/adrenaline, inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), plasminogen activator inhibitor-1 (PAI-1), intercellular adhesion molecule-1 (ICAM-1), glycated hemoglobin (HbA1c), and insulin were measured using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one portion was referred as the untreated test formulation, while the other portion and three groups of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by a renowned spiritual healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of adrenaline was reduced by 29.25%, 20.33%, 31.36%, and 41.97% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), and G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the untreated test formulation group (G4). Moreover, the level of iNOS was reduced by 53.09%, 51.32%, 33.87%, 39.88%, and 40.02% in the G5, G6, G7, G8, and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the disease control group (G2). Additionally, the level of eNOS was increased by 83.65%, 94.93%, 71.48%, 48.64%, and 61.12% in the G5, G6, G7, G8, and G9 groups, respectively, as compared to the G2 group. The level of PAI-1 was decreased by 56.10%, 56.57%, 55.28%, 49.28%, and 57.84% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Besides, the level of ICAM-1 was decreased by 20.99%, 20.74%, 18.85%, and 17.89% in the G5, G6, G7, and G8 groups, respectively as compared to the G2 group. Level of HbA1c was decreased by 28.50%, 44.07%, 25.30%, 22.70%, and 48.79% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Further, the level of insulin was decreased by 26.09%, 21.24%, 5.41%, 23.61%, and 24.59% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance improvement of vital functional biomarkers of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).
Trivedi MK and Jana S
<a href="https://medwinpublishers.com/AII/volume.php?volumeId=366&issueId=1199">https://medwinpublishers.com/AII/volume.php?volumeId=366&issueId=1199</a>
Medwin Publishers
5-Feb-2021
English
Journal Article