Skin health and aging are the complex biological process influenced by several intrinsic (or endogenous) and extrinsic (or exogenous) factors. Various skin-based therapies are currently available to rejuvenate the skin, but they might be related with some side-effects such as scarring. The objective of the present study was to evaluate the effect of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the human foreskin fibroblast (HFF-1) cell line and Dulbecco’s Modified Eagle Medium (DMEM) for skin health parameters like cell proliferation and synthesis of collagen. The rate of cellular proliferation in HFF-1 cells was identified, and the results found that the Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased by 152.38% compared to the negative control group. Additionally, the cell proliferation was also significantly increased by 71.43% in the Biofield Energy Treated cells compared to the negative control group. Similarly, the collagen level was significantly (p ≤ 0.001) increased by 60.42% in the Biofield Energy Treated DMEM compared with the negative control group. Hence, the results exhibited a significant improvement of collagen synthesis and cellular proliferation in the Biofield Energy Treated DMEM for improving skin health. It can be concluded that The Trivedi Effect® - Consciousness Energy Healing Treatment might be a complementary and alternative approach with respect to the skin health, anti-aging in DMEM compared with the HFF-1 cell line. Therefore, the Biofield Energy Treated DMEM could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles, etc.
Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p≤0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line.