Antiaging Potential of Consciousness Energy Healing-Based Novel Proprietary Formulation
Antiaging
<p align="justify">The study objective was to evaluate the potential of Biofield Treatment on novel proprietary formulation for skin health using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part received Biofield Treatment by Gopal Nayak and defined as Biofield Treated (BT) sample, while other part was denoted as untreated (UT) sample. MTT assay showed more than 70% cell viability, suggested the test formulation were safe and nontoxic. BrdU data showed an increased cell proliferation by 170%, 170%, and 212% at 0.1 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated group. Collagen was significantly increased by 140.8% at 5 µg/mL in UT-DMEM + BT-Test formulation than untreated. Elastin was significantly increased by 233.3%, 313.3%, and 637.8% at 0.01 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated. Hyaluronic acid in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation was significantly increased by 191.8%, 461.0%, and 322.5%, respectively at 0.01 µg/mL than untreated. Melanin synthesis was significantly inhibited by 44.7% (10 µg/mL) and 45.2% (10 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively contrast to untreated. Anti-wrinkling activity was improved with increased cell viability by 88.9% and 92.9% at 1 and 25 µg/mL, respectively in BT-DMEM + UT-Test formulation than untreated. Cell viability was significantly increased by 97.6% and 75.16% at 0.1 and 1 µg/mL, respectively in BT-DMEM + BT-Test formulation than untreated. The UT-DMEM + BT-Test formulation showed increased cellular migration by 2722.2% (0.01 µg/mL) in HaCaT cells than untreated. Overall, result suggests that the Trivedi Effect® has shown significant beneficial effects on skin health parameters that might lead to its potential use in various aging-related disorders, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.</p>
<p align="justify"></p>
Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
<a href="https://gavinpublishers.com/articles/Research-Article/Food-Nutrition-Journal-ISSN-2575-7091/Antiaging-Potential-of-Consciousness-Energy-Healing-Based-Novel-Proprietary-Formulation" target="_blank" rel="noreferrer noopener">https://gavinpublishers.com/articles/Research-Article/Food-Nutrition-Journal-ISSN-2575-7091/Antiaging-Potential-of-Consciousness-Energy-Healing-Based-Novel-Proprietary-Formulation</a>
Gavin Publishers
05 October, 2018
English
Journal Article
10.29011/2575-7091.100090
Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health
Skin-Health
<p style="text-align:justify;">Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.</p>
Jagdish Singh, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=394&doi=10.11648/j.ajpp.20170201.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=394&doi=10.11648/j.ajpp.20170201.11</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ajpp.20170201.11
Evaluation of Anti-aging Effect of the Novel Test Formulation in Cell-based Studies using Β-Galactosidase Activity, Collagen Levels, and Protection against Oxidative Stress
Health & Wellness
The study was aimed to evaluate the antioxidant and antiaging potential of Biofield Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in in vitro. The test formulation was divided into two parts. One part was denoted as the control, without any Biofield Treatment, while the other part was defined as the Biofield Energy Treated test formulation. MTT assay showed that the test formulation was observed as safe upto 100 μg/mL in both human foreskin fibroblasts-1 (HFF-1) and mouse preadipocytes (3T3-L1) cells. Moreover, the Biofield Treated test formulation showed 35.73% and 122.07% increased cellular protection (cell viability) at 25 and 50 μg/mL, respectively in H2O2 induced oxidative damage as compared to the untreated. Collagen was significantly increased by 16.81% and 30.22% in the Biofield Treated test formulation at 75 and 100 μL, respectively as compared to the untreated. Further, cellular senescence was significantly reduced by 66.35% and 22.5% in the Biofield Treated test formulation at 50 and 100 μg/mL, respectively compared to the untreated. Overall, data suggested that the Biofield Energy Treated test formulation can significantly improve antioxidative properties and would be use against antiaging, autoimmune and inflammatory diseases, stress management and prevention by improving overall health.
Trivedi MK and Jana S
<a href="http://www.annexpublishers.com/journals/journal-of-cell-biology-and-histology/articles.php?volume=2&issue=1">http://www.annexpublishers.com/journals/journal-of-cell-biology-and-histology/articles.php?volume=2&issue=1</a>
Annex Publishers
20/Dec/2020
English
Journal Article
Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula
Skin-Health
<p style="text-align:justify;">Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.</p>
William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20170503.13" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20170503.13</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.ajhr.20170503.13
Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters
Skin-Health
<p style="text-align:justify;">The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.</p>
Janice Patricia Kinney, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170502.15" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170502.15</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ajls.20170502.15
Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters
Skin-Health
<p style="text-align:justify;">The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.</p>
Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170503.11">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170503.11</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ajls.20170503.11
Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it’s implication for wound healing
Nutraceuticals
<p style="text-align:justify;">The aim of this study was to investigate the role of tetrahydrocurcumin (THC) against various skin health parameters using in vitro human foreskin fibroblast and melanoma cell lines (i.e. HFF-1 and B16-F10). The study was assessed using cell viability by MTT assay, identification of extracellular matrix component in HFF-1 cell line (i.e. collagen, elastin and hyaluronic acid), melanin synthesis in B16-F10 cells, cell viability against UVB-induced stress in HFF-1 cells, and in vitro wound healing by the scratch assay. THC was found to be safe and nontoxic up to the concentration of 10 µg/mL with improved level of collagen (37.90%), elastin (90.1%), and hyaluronic acid (74.19%) at 1 µg/mL. Besides, melanin was significantly inhibition by 78.5% at the lowest THC concentration of 0.1 µg/mL. UVB-protection rate was significantly improved by 61.2% and improved cell viability by THC in HFF-1 cells, which indicated protection from photoaging. In addition, THC showed significant wound healing activity (78.51%) and greater migration of fibroblast in HFF-1 cells at different time period. It can be concluded from the study that THC can protect the skin matrix with improved extracellular component synthesis and would healing via collagen synthesis in the skin, which improved the skin elasticity and tightness. Overall, it might be suggested that THC can be used as a safe skin whitening agent, wounds management, cosmetic applications, and treating various skin-related disorders.</p>
Mahendra Kumar Trivedi, Mayank Gangwar, Sambhu Charan Mondal, Snehasis Jana
<a href="https://link.springer.com/article/10.1007/s13197-017-2525-8" target="_blank" rel="noreferrer noopener">https://link.springer.com/article/10.1007/s13197-017-2525-8</a>
Spiringer Link
February 27, 2017
English
Journal Article
10.1007/s13197-017-2525-8
Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.</p>
Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=651&doi=10.11648/j.ejpm.20170502.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=651&doi=10.11648/j.ejpm.20170502.11</a>
Science Publishing Group
May 03, 2017
English
Journal Article
10.11648/j.ejpm.20170502.11
Skin Photo-Protective and Anti-ageing Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">The objective of this study was to evaluate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment based herbomineral formulation on various skin parameters using HFF-1, HaCaT, and B16-F1 cell lines. The test formulation was composed of minerals (zinc, selenium, and molybdenum), and L-ascorbic acid along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). Test formulation and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment by Bonnie P. Hegarty-Diaz and denoted as treated, while other part was coded as untreated groups. MTT assay result showed that the test formulation was found safe and nontoxic at tested concentration 40 µg/mL. Fibroblast cell proliferation assay showed significant increased cell proliferation by 26.83% at 8.75 µg/mL in BT-DMEM + BT-Test formulation group compared to the untreated group. The level of collagen was significantly increased by 22.05% and 22.24% (at 2.5 µg/mL) in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively. However, collagen amount was increased by 11.08% and 9.52% in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL, compared to untreated group. Similarly, elastin synthesis was increased in the BT-DMEM + UT-Test formulation group by 19.98%, 72.54% and 105.04% at the concentrations 10, 5, and 2.5 µg/mL, respectively compared to the untreated group. However, level of hyaluronic acid was increased by 5.19% at 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with the untreated group. Besides, melanin synthesis inhibition was found to be inhibited by 14.80% and 17.40% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed improve cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 10.69%, 6.9%3, and 11.86%, respectively at 0.625 µg/mL compared with the untreated group. In addition, wound healing scratch assay data suggest significantly higher cellular migration of fibroblast and keratinocytes cells in HFF-1 and HaCaT cells lines, respectively. In conclusion, Biofield Energy Healing (The Trivedi Effect®) based test formulation and DMEM could be beneficial against various skin disorders such psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections and can be significantly used as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action.</p>
Bonnie P. Hegarty-Diaz, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=320&doi=10.11648/j.jdmp.20170303.12" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=320&doi=10.11648/j.jdmp.20170303.12</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.jdmp.20170303.12
Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.</p>
Dennille Mellesia Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.15" target="_blank" rel="noreferrer noopener">http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.15</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.jfns.20170503.15