Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation
Health & Wellness
The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constituents were divided into two parts; one section was defined as untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing/Blessing Treatment and divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range from 73% to 307%. Young’s modulus (YM) is a measure of cell stiffness, a decrease in YM value indicates increase elasticity of the cells and vice-versa. YM in H9C2 cells were decreased by 9.6% and 66.1% in the BT-DMEM + UT-TI group at 0.1 and 1 μg/mL respectively, as compared with untreated test group. However, C2C21 cells showed increased YM by 443.9% at 1 μg/mL in the UT-DMEM + BT-TI group, while 869.6% increased YM in the BT-DMEM + UT-TI group at 1 μg/mL as compared with untreated test group. However, 314% increased YM was reported in the BT-DMEM + BT-TI group at 1 μg/mL as compared with the untreated test group. However, the value of YM was significantly decreased in the HaCaT cell line by 247.7% (at 1 μg/mL), 225.8% (at 0.1 μg/mL), and 97.9% (at 1 μg/mL) in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. In addition, YM was significantly decreased in the SH-SY5Y cell line by 92.6%, 18.1%, and 26.6% at 1 μg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. Overall, the results showed the significant decreased YM among the SH-SY5Y, HaCaT, and H9C2 cells, while it was increased in the C2C21 cell line. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with improved cellular elasticity and its associated complications/symptoms.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
<a href="https://openaccesspub.org/jbbs/article/1662">https://openaccesspub.org/jbbs/article/1662</a>
Openaccesspub
13-Jul-2021
English
Journal Article
Antiaging Potential of Consciousness Energy Healing-Based Novel Proprietary Formulation
Antiaging
<p align="justify">The study objective was to evaluate the potential of Biofield Treatment on novel proprietary formulation for skin health using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part received Biofield Treatment by Gopal Nayak and defined as Biofield Treated (BT) sample, while other part was denoted as untreated (UT) sample. MTT assay showed more than 70% cell viability, suggested the test formulation were safe and nontoxic. BrdU data showed an increased cell proliferation by 170%, 170%, and 212% at 0.1 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated group. Collagen was significantly increased by 140.8% at 5 µg/mL in UT-DMEM + BT-Test formulation than untreated. Elastin was significantly increased by 233.3%, 313.3%, and 637.8% at 0.01 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated. Hyaluronic acid in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation was significantly increased by 191.8%, 461.0%, and 322.5%, respectively at 0.01 µg/mL than untreated. Melanin synthesis was significantly inhibited by 44.7% (10 µg/mL) and 45.2% (10 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively contrast to untreated. Anti-wrinkling activity was improved with increased cell viability by 88.9% and 92.9% at 1 and 25 µg/mL, respectively in BT-DMEM + UT-Test formulation than untreated. Cell viability was significantly increased by 97.6% and 75.16% at 0.1 and 1 µg/mL, respectively in BT-DMEM + BT-Test formulation than untreated. The UT-DMEM + BT-Test formulation showed increased cellular migration by 2722.2% (0.01 µg/mL) in HaCaT cells than untreated. Overall, result suggests that the Trivedi Effect® has shown significant beneficial effects on skin health parameters that might lead to its potential use in various aging-related disorders, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.</p>
<p align="justify"></p>
Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
<a href="https://gavinpublishers.com/articles/Research-Article/Food-Nutrition-Journal-ISSN-2575-7091/Antiaging-Potential-of-Consciousness-Energy-Healing-Based-Novel-Proprietary-Formulation" target="_blank" rel="noreferrer noopener">https://gavinpublishers.com/articles/Research-Article/Food-Nutrition-Journal-ISSN-2575-7091/Antiaging-Potential-of-Consciousness-Energy-Healing-Based-Novel-Proprietary-Formulation</a>
Gavin Publishers
05 October, 2018
English
Journal Article
10.29011/2575-7091.100090
The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies
Skin-Health
<p style="text-align:justify;">The present study was undertaken to evaluate the effect of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 75% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The percent cell proliferation by BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 8.75 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Elastin was significantly (p≤0.001) increased 93.52% and 75.81% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Hyaluronic acid was significantly increased by 100.07% (p≤0.05), 41.60%, and 50.10% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 0.625 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Melanin was decreased by 10.09% and 6.51% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 0.125 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased by 7.22%, 5.75%, and 8.15% in the BT-DMEM + BT-Test Formulation at 0.625%, 1.25%, and 2.5% µg/mL, respectively compared to the UT-DMEM + UT-Test Formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 cells compared to the UT-DMEM + UT-Test formulation group. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better skin protection action compared to the untreated DMEM and test formulation. Therefore, the Biofield Energy Treated test herbomineral formulation could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.</p>
Vaibhav Rajan Parulkar, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.16" target="_blank" rel="noreferrer noopener">http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.16</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.jfns.20170503.16
Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.</p>
Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=651&doi=10.11648/j.ejpm.20170502.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=651&doi=10.11648/j.ejpm.20170502.11</a>
Science Publishing Group
May 03, 2017
English
Journal Article
10.11648/j.ejpm.20170502.11
The Study of Biofield Energy Treatment Based Herbomineral Formulation in Skin Health and Function
Skin-Health
<p style="text-align:justify;">The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines. It contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts each, one part of each was coded as untreated (UT), while the second part of each was treated with the Biofield Treatment by Johanne Dodon and denoted as the Biofield Energy Treated (BT) test items. MTT assay result showed that the test formulation was safe and nontoxic up to 80 µg/mL concentration. The fibroblast cell proliferation assay using BrdU method showed a significant increased cell proliferation at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54%, respectively in the BT-DMEM + BT-Test formulation group compared to the untreated group. The collagen data showed an increased synthesis by 7.42% and 3.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Similarly, the elastin synthesis was increased by 19.8% and 25.8% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL with respect to the untreated group. However, the Biofield Energy Treated test formulation showed an increased level of hyaluronic acid by 1.7% in UT-DMEM + BT-Test formulation group compared to the untreated group. Melanin synthesis inhibition was observed by 15.21% and 11.43% in UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation groups, respectively at 0.125 µg/mL compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling effects showed an improved cell viability by 7.65% at 1.25 µg/mL in the BT-DMEM + BT-Test formulation experimental group compared with the untreated test group. In addition, the Biofield Energy Treated Test formulation/DMEM showed a significant wound healing activity and high cellular migration of fibroblast and keratinocytes in HFF-1 and HaCaT cells, respectively. Overall, the data suggested that Biofield Treated formulation/DMEM could be helpful for skin healing, improvement the skin health, elasticity, and tightness, as well as, improving skin health issues such as wrinkling, pigmentation disorders, redness, swelling, skin cancer, aging, rashes, and skin sagging.</p>
Johanne Dodon, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20170503.12" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=219&doi=10.11648/j.ajbio.20170503.12</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.ajbio.20170503.12
Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters
Skin-Health
<p style="text-align:justify;">The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.</p>
Janice Patricia Kinney, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170502.15" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20170502.15</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ajls.20170502.15
Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.</p>
James Jeffery Peoples, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=612&doi=10.11648/j.ajpb.20170203.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=612&doi=10.11648/j.ajpb.20170203.11</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.ajpb.20170203.11
Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health
Skin-Health
<p style="text-align:justify;">Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.</p>
Jagdish Singh, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=394&doi=10.11648/j.ajpp.20170201.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=394&doi=10.11648/j.ajpp.20170201.11</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ajpp.20170201.11
Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula
Skin-Health
<p style="text-align:justify;">Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.</p>
William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20170503.13" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=656&doi=10.11648/j.ajhr.20170503.13</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.ajhr.20170503.13
Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation
Skin-Health
<p style="text-align:justify;">The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.</p>
Dennille Mellesia Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.15" target="_blank" rel="noreferrer noopener">http://sciencepublishinggroup.com/journal/paperinfo?journalid=154&doi=10.11648/j.jfns.20170503.15</a>
Science Publishing Group
May 09, 2017
English
Journal Article
10.11648/j.jfns.20170503.15