A new proprietary herbomineral formulation was formulated, consisting of an essential ingredients viz. an herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of this study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed in this experiment. The humoral immune response data showed the secondary antibody titre was significantly increased by 112.50% and 87.50% in the Biofield Energy Treated test formulation group (G4) and untreated test formulation group (G5) compared to the disease control group (G2). The results of delayed type hypersensitivity showed significant increased the paw volume by 111.76%, 51.45%, 100%, and 64.80% in the G4, G5, Biofield Energy Treatment group per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7) groups, respectively compared to the G2 group. The platelet count was significantly increased by 37.72%, 8.69%, 16.30% and 33.11% in the G4, G5, G6, and G7 groups, respectively compared to the G2 group. The level of blood urea nitrogen was significantly decreased by 14.01% in the G4 group compared to the disease control. Moreover, the concentration of uric acid was significantly reduced by 28.97% in the G7 group compared to the G2 group. Animal weight parameters suggested that there were no treatment-related changes in any group. Organ to body weight ratio, feed and water intake data described that the Biofield Energy Treated test formulation was found to be safe without any side-effect during the course of the experiment. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
The use of herbomineral formulations for different chronic diseases is gaining popularity because these natural formulations have fewer side effects, a higher safety profile, and more cost effectiveness. A new proprietary herbomineral formulation composed of herbs and certain minerals was formulated, consisting of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of a Biofield Energy Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in female Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. Immunomodulatory potential, humoral immune response, paw volume, hematological study, biochemistry, body weight, feed intake and histopathology analysis were performed. Humoral immune response data showed increased (p≤0.01) primary antibody titre by 124.7% in the Biofield Treatment test formulation (G3), while the untreated test formulation group (G4) showed decreased antibody titre compared with the disease control group (G2). The secondary antibody titre value in the G3 group was also altered compared with the G2 group. Delayed type hypersensitivity (DTH) data suggest that the Biofield Energy Treated test formulation group (G3) showed a significant decrease in paw volume by 128.57% with respect to the G2 group. Hematology and biochemistry analysis showed altered values, as increased MCHC and RBC count by 138.09% and 7.24%, respectively in the G3 group compared with the G2 and G4 group. However, the platelet count was increased by 17.70% in the G3 group compared with the G2 group. The level of blood urea nitrogen was decreased by 10.90% in the G3 level compared with the G2 group. However, the levels of magnesium and phosphorus were increased by 13.29% and 6.28%, respectively in the G3 compared with the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulations was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with enhancing stress management and anti-aging by improving overall health.
Herbomineral formulations have been used worldwide against various chronic and degenerative diseases due to its fewer side effects. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. Biomarkers like immunoglobulins (IgG, IgM), cluster differentiation (CD4+), CD8+, superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO) were monitored. The level of IgM was increased by 4.76% in the Biofield Energy Treated test formulation (G4) compared to the disease control group (G2). The levels of CD4+ and CD8+ counts were significantly (p≤0.01) increased by 222.22% and 355.36% in the G4 group compared to the G2 group. The level of lymphocyte was increased by 5% and eosinophil count was significantly decreased by 75% in the G4 group compared to the G2 group. The lipid biomarkers such as total cholesterol (TC), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were significantly lowered by 9.70%, 6.67%, and 23.54%, respectively in the G4 group compared to the G2 group. The expression of SOD was reduced by 9.96% in the G4 group compared to the G2 group. Further, LPO expression was significantly reduced by 33.38% and 16.88% in the G4 and untreated test formulation (G5) groups, respectively compared to the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed significantly improved the cellular and humoral immunity, hematological and biochemical profile compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
The natural products based formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with four ingredients, a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematological parameters, lipid profile, hepatic enzymes, and the sex hormone (testosterone). The ratio of CD4+/CD8+ was significantly increased by 51.98% and 12.99% (p≤0.05) in the Biofield Energy Treated (G3) and untreated test formulations (G4), respectively compared with the disease control group (G2). The level of IgM was significantly increased by 28.57% in the G3 group, while decreased by 14.29% in the G4 group compared with the G2 group. The eosinophil count was significantly increased by 27.27% in the G3 group, while decreased by 18.18% in the G4 group compared with the G2 group. The levels of triglycerides (TG) and very low density lipoproteins (VLDL) were reduced by 8.99% and 9.12%, respectively in the G3 group compared with the G2 group. However, the levels of TG and VLDL were reduced by 2.40% and 3.46%, respectively in the G4 group compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of serum glutamate oxaloacetate transaminase (SGOT) and creatine kinase myocardium band (CK-MB) by 42.65% (p<0.01) and 7.67%, respectively in the Biofield Energy Treated test formulation group (G3) compared to the G2 group. The level of testosterone was significantly increased by 22.02% in the G3 group compared with G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
The herbomineral formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in female Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The Biofield Energy Treated group (G3) showed 6.39% increased the CD4+ count compared with the disease control (G2) group. The level of IgM was significantly increased by 23.08% and 46.15% in the G3 group and Biofield Energy Treatment group per se at day -15 (G6), respectively compared with the G2 group. The total leucocyte count (TLC) and neutrophil were significantly increased by 16.15% and 26.69% (p≤0.05), respectively in the G3 group compared with the G2 group. Further, the levels of TLC, eosinophil, and monocyte were significantly increased by 70.57% (p≤0.01), 33.33%, and 8.50%, respectively in the G6 group compared with the G2 group. Low density lipoprotein (LDL) was reduced by 6.24% in the G3 group, while triglyceride (TG) and LDL were reduced by 12.12% and 16.99%, respectively in the G6 group compared with the G2 group. The levels of serum glutamate-pyruvate transaminase (SGPT), alkaline phosphatase (ALP), and total bilirubin were significantly decreased by 20.26%, 32.98%, and 25.00%, respectively in the G3 group compared with the G2 group. Further, SGPT and total bilirubin were reduced by 9.02% and 16.67%, respectively in the G6 group compared with the G2 group. Progesterone was significantly increased by 59.85% in the G3 group, compared with the G2 group. The data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment group per se at day -15 have shown a significant immunomodulatory potential. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data also suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) based herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory potential was studied in male SD rats using various immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response showed an increase IgG level by 6.10% in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Cellular immune response i.e. CD4+ and CD8+ counts were significantly improved by 51.64% and 52.09%, respectively in G4 compared with the G2 group. The lymphocytes level were increased by 38.87%, while TLC, neutrophils, eosinophils, and monocytes count were decreased by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in G4 compared with the G2 group. Lipid analysis showed that triglycerides and VLDL levels were decreased by 6.02% and 5.97%, respectively while HDL level was significantly increased by 28.44% in G4 compared with the G2 group. Hepatic biomarkers viz. SGOT, CK-MB, total protein, total bilirubin, and globulin by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in G4 compared with the G2 group. However, the testosterone level was significantly increased in G4 and untreated test formulation (G5) groups by 135.74% and 28.09%, respectively compared with the G2 group. Additionally, the antioxidant assay showed that the lipid peroxidation (LPO) level was decreased, while superoxide dismutase (SOD) and catalase levels were increased by 59.72% and 20.07%, respectively in the G4 compared with the G2 group. Overall, the study results suggested that the Biofield Energy Treated test formulation showed an improved cellular and humoral immune responses along with improved hematological and biochemical animal profile compared with the untreated test formulation. Consequently, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
The use of an herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side-effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal (ashwagandha root extract) and minerals (zinc chloride, magnesium gluconate, and sodium selenate). The objective of this study was to evaluate the immunomodulatory effect of the Biofield Energy Treated test formulation on female Sprague Dawley (SD) rats. The test formulation was divided into two parts, one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Energy Treated formulation. The effect of the test formulation was monitored using humoral immune response, delayed type hypersensitivity, hematology, biochemistry, relative organ weight, and histopathology. The primary hemagglutination (HA) antibody titre level was significantly (p≤0.05) increased by 185.88% in the Biofield Energy Treated group (G3), while 41.18% increased in the untreated test formulation group (G4) as compared to the disease control group (G2). The secondary HA titre was significantly increased by 540% (p≤0.001) and 166.7% in the G3 and G4 groups, respectively as compared to the G2 group. The secondary HA titre was also increased significantly (p≤0.05) by 193% in the Biofield Energy Treated group per se at day -15 (G6), as compared to the G2 group. The paw volume data showed an alteration in the G3 and G6 groups as compared to the G2 group. The platelet count was significantly increased by 11.16% and 8.69% in the G3 and G4 groups, respectively as compared to the G2 group. The magnesium level was increased by 9.31% in the G3 group as compared to the G2 group. The uric acid was significantly reduced by 23.88% in the G3 group, while it was increased by 17.47% in the G4 group as compared to the G2 group. Since, the relative organ weight and histopathological findings did not produce any signs of toxicity as compared to the normal control group (G1). These data suggested that the Biofield Energy Treated herbomineral formulation was found to be safe and showed better immunomodulatory response as compared to the untreated formulation. Thus, it is assumed that the Biofield Energy Treated herbomineral formulation could be better and more useful to boost-up the immune system in healthy human and patients.
Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
Herbomineral formulations are used world-wide for various therapeutic purposes. More than 80% of the world population relies on natural herbal and mineral remedies as medicines for their health care. A new proprietary herbomineral formulation was created, consisting of the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of the Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and the untreated test formulations were studied to determine any alterations in the animal humoral immune response, paw volume, hematological study, serum biochemistry parameters, animal weight parameters, feed intake and histopathology analysis. Humoral immune response data of the reference standard (levamisole) showed a significant increase in secondary antibody titre value (p≤0.05), while the Biofield Treated test formulation group (G3) exhibited a significant increase in the secondary antibody titre by 115.34% compared with the disease control group (G2). A delayed type hypersensitivity (DTH) reaction showed that the paw volume was significantly decreased by 114.28% in the Biofield Energy Treated test formulation group (G3) with respect to the G2 group. The platelets count was increased by 61.55% in the Biofield Treated test formulation group (G3) compared to the G2 group. Moreover, the administration of the Biofield Treated herbomineral formulation (G3) group exhibited a decrease in the level of creatinine (9.62%), and uric acid (14.40%), while the level of potassium ion concentration was incresaed by 77.43% compared to the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulation was found to be safe. Overall, The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) significantly modulates the immunological parameters of the herbomineral formulation compared with the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases along with stress management and anti-aging by improving overall health.
Due to the increased popularity of herbomineral preparations in the healthcare sector, a new proprietary herbomineral formulation was formulated consisting of ashwagandha root extract and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells. The herbomineral formulation was divided into two parts, one defined as the control, while the other part was treated with the Biofield Energy Healing Treatment performed from a remote distance by twenty renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The splenocyte cells were exposed to test formulations at concentration from 0.00001053 to 10.53 µg/mL and were analyzed after 48 hours for cell viability using MTT assay. The expression of the cytokines (TNF-α, IFN-γ, IL-1β, and MIP-1α) was determined using ELISA assay. The cell viability data showed that all the tested concentration ranges were found to be safe with percentage cell viability at more than 80%. Further, TNF-α expression was significantly inhibited in the Biofield Treated test formulation group with respect to the vehicle control, while at 0.001053 and 0.1053 µg/mL, the expression was suppressed by 1.70% and 8.16%, respectively in the Biofield Treated test formulation compared to the untreated formulation. However, a significant immunosuppression was reported in IFN-γ expression at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The MIP-1α expression was inhibited by 8.31%, 21.53%, and 8.70% at 0.0001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated formulation compared with the untreated test formulation. However, IL-1β expression was significantly suppressed by 19.72% at concentration 0.00001053 µg/mL in the Biofield Treated test formulation compared with the untreated test formulation. Thus, the down-regulation of tested cytokines and chemokines in the Biofield Energy Healing test formulation might be applicable for controlling acute and chronic inflammation in many clinical diseases. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory activity of the test formulation, which can be useful against autoimmune disorders. Biofield Treated Test formulation may also be useful in anti-aging, anti-inflammatory, stress management and in preventing immune-mediated tissue damage in organ transplants by improving overall health and quality of life.