Evaluation of Immune Biomarkers After Oral Administration of the Novel Herbomineral Formulation Treated with The Trivedi Effect® - Biofield Energy Healing in Male Sprague Dawley Rats
nutraceuticals
<p style="text-align:justify;">Herbomineral formulations have been used worldwide against various chronic and degenerative diseases due to its fewer side effects. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. Biomarkers like immunoglobulins (IgG, IgM), cluster differentiation (CD4+), CD8+, superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO) were monitored. The level of IgM was increased by 4.76% in the Biofield Energy Treated test formulation (G4) compared to the disease control group (G2). The levels of CD4+ and CD8+ counts were significantly (p≤0.01) increased by 222.22% and 355.36% in the G4 group compared to the G2 group. The level of lymphocyte was increased by 5% and eosinophil count was significantly decreased by 75% in the G4 group compared to the G2 group. The lipid biomarkers such as total cholesterol (TC), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were significantly lowered by 9.70%, 6.67%, and 23.54%, respectively in the G4 group compared to the G2 group. The expression of SOD was reduced by 9.96% in the G4 group compared to the G2 group. Further, LPO expression was significantly reduced by 33.38% and 16.88% in the G4 and untreated test formulation (G5) groups, respectively compared to the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed significantly improved the cellular and humoral immunity, hematological and biochemical profile compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=254&doi=10.11648/j.ajcem.20170506.15" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=254&doi=10.11648/j.ajcem.20170506.15</a>
Science Publishing Group
December 11, 2017
English
Journal Article
10.11648/j.ajcem.20170506.15
Evaluation of Immune Biomarkers After Oral Administration of Biofield Energy Healing Based Herbomineral Formulation in Male Sprague Dawley Rats
Nutraceuticals
<p style="text-align:justify;">The natural products based formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with four ingredients, a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematological parameters, lipid profile, hepatic enzymes, and the sex hormone (testosterone). The ratio of CD4+/CD8+ was significantly increased by 51.98% and 12.99% (p≤0.05) in the Biofield Energy Treated (G3) and untreated test formulations (G4), respectively compared with the disease control group (G2). The level of IgM was significantly increased by 28.57% in the G3 group, while decreased by 14.29% in the G4 group compared with the G2 group. The eosinophil count was significantly increased by 27.27% in the G3 group, while decreased by 18.18% in the G4 group compared with the G2 group. The levels of triglycerides (TG) and very low density lipoproteins (VLDL) were reduced by 8.99% and 9.12%, respectively in the G3 group compared with the G2 group. However, the levels of TG and VLDL were reduced by 2.40% and 3.46%, respectively in the G4 group compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of serum glutamate oxaloacetate transaminase (SGOT) and creatine kinase myocardium band (CK-MB) by 42.65% (p<0.01) and 7.67%, respectively in the Biofield Energy Treated test formulation group (G3) compared to the G2 group. The level of testosterone was significantly increased by 22.02% in the G3 group compared with G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=246&doi=10.11648/j.ijbbmb.20170206.15" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=246&doi=10.11648/j.ijbbmb.20170206.15</a>
Science Publishing Group
December 11, 2017
English
Journal Article
10.11648/j.ijbbmb.20170206.15
Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats
nutraceuticals
<p style="text-align:justify;">Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=244&doi=10.11648/j.bmb.20170206.17" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=244&doi=10.11648/j.bmb.20170206.17</a>
Science Publishing Group
December 11, 2017
English
Journal Article
10.11648/j.bmb.20170206.17
Evaluation of the Effects of Biofield Energy Healing Based Herbomineral Formulation on Various Biomarkers in Male Sprague Dawley Rats: Potential Role of the Trivedi Effect®
Nutraceuticals
<p style="text-align:justify;">A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The IgM, IgG, CD4+, and CD8+ were altered in the Biofield Energy Treated test formulation (G4) compared to the disease control (G2). TLC was significantly increased by 19.35% in the G4 compared to the G2. Neutrophil was significantly increased by 31.53%, 29.73%, 31.53%, and 33.84% in the G4, untreated test formulation (G5), G6, and G7, respectively compared to the G2. The levels of total cholesterol (TC), triglycerides (TG), and very low density lipoprotein (VLDL) were significantly lowered by 18.18% (p≤0.01), 46.33% (p≤0.01), and 46.41% (p≤0.01), respectively in the G4 compared to the G2. Additionally, the levels of TC, TG, low density lipoprotein (LDL), and VLDL were significantly reduced by 25.50% (p≤0.001), 96.39% (p≤0.001), 13.91%, and 55.08% (p≤0.001), respectively in the G6 compared to the G2. Alkaline phosphatase (ALP) was significantly reduced by 33.65% (p≤0.001) in the G4 compared to the G2. Moreover, the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), ALP, and creatine kinase myocardium band (CK-MB) were significantly decreased by 29.44% (p≤0.05), 25.54% (p≤0.01), 27.97% (p≤0.01), and 36.07% (p≤0.01), respectively in the G7 group compared to the G2. Testosterone was significantly increased by 83.68%, 305.90%, 1271.03% (p<0.001) and 745.35% (p<0.05) in the G4, G5, G6 and G7, respectively compared to the G2. The level of catalase enzyme was significantly increased by 57.91% (p<0.01) and 98.51% (p<0.001) in Biofield Energy Treatment per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7), respectively compared to the G2. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=330&doi=10.11648/j.ijpc.20170306.16" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=330&doi=10.11648/j.ijpc.20170306.16</a>
Science Publishing Group
December 05, 2017
English
Journal Article
10.11648/j.ijpc.20170306.16