The current research article was aimed to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The liquid chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the Trivedi Effect® Treated sample displayed these peaks at Rt of 1.79 and 2.03 min. The ESI-MS spectra of the control and the Trivedi Effect® Treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered as compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two magnesium gluconate ion forms at m/z 447 and 415 in treated sample was significantly decreased by 59.82% and 55.44%, respectively compared with the control sample. The percentage change in the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably decreased in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% compared with the control sample. Consequently, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Thus, the Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergies, aging, inflammatory diseases, immunological disorders, and other chronic infections. The treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
The current research work was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the structural properties and isotopic abundance ratio (PM+1/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts. One part was denoted as the control, while the another part was defined as The Trivedi Effect® Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The total ion chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the treated sample displayed peaks at Rt of 1.79 and 2.04 min. The ESI-MS spectra of the control and the treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and treated samples. The LC-MS based isotopic abundance ratio analysis indicated that the PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated magnesium gluconate ion at m/z 415 was significantly decreased by 79.24% compared with the control sample. Similarly, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the gluconate ion was decreased by 6.62% in the treated sample compared with the control sample. The treated magnesium gluconate might be beneficial in the nutraceutical and/or pharmaceutical industries for designing various forms of formulations, which could be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. Consequently, The Trivedi Effect® - Energy of Consciousness Healing Treated magnesium gluconate would be more helpful for understanding the enzymatic reactions as well as assist in the designing of the novel potent enzyme inhibitors by applying its kinetic isotope effects.
The current research work was designed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416, 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-α, 6α-epoxy-β, 5β, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4β, 5β, 6α, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect® treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of - 4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4-dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy-γ-butyrolactone (3) was decreased by - 14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect® - Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.
Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416, 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.
The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) and PM+2/PM (18O/16O or 26Mg/24Mg) were significantly decreased in the treated sample by 35.97% and 66.77%, respectively compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416, 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly reduced compared with the control sample. Thus, The Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.
Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416, 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
Withania somnifera (Ashwagandha) root extract is very useful herbal medicine since from ancient time. The current study designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural properties of the ashwagandha root hydroalcoholic extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area % at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.3, 5.5, 6.4, 6.5, 6.8, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, 8.2, 8.4, 8.6, 8.8, 9.0, 9.1, and 10.1 minutes were significantly reduced by 0.57% to 38.10% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.7, 6.7, and 8.1 minutes were significantly increased by 16.00%, 244.44% and 19.62%, respectively compared with the control sample. A total of 21 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 569, 621, 489, 473, 767, 471, 505, 992, and 455 at retention times of 6.4, 6.5, 6.8, 7.1, 7.9, 8.1, 8.4, 9.1, and 10.1 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. There were significant changes observed in the peak intensity values at the same retention time in the Biofield Energy Treated sample in the range of -75.32% to 108.51% compared with the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.