Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa
Microbial Genetics
<p style="text-align:justify;">Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics. Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data. Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/antibiogram-biochemical-reactions-and-genotypic-pattern-of-biofield-treated-pseudomonas-aeruginosa-2329-891X-1000181.php?aid=65292" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/antibiogram-biochemical-reactions-and-genotypic-pattern-of-biofield-treated-pseudomonas-aeruginosa-2329-891X-1000181.php?aid=65292</a>
Omics Publishing Group
October 07, 2015
English
Journal Article
10.4172/2329-891X.1000181
Impact of an external energy on Enterococcus faecalis [ATCC – 51299] in relation to antibiotic susceptibility and biochemical reactions – An experimental study
Microbiology
<p style="text-align:justify;">Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists Results: The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy/treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.</p>
Mahendra kumar Trivedi, Dr. Yogi Bhardwa, Shrikant Patil, Harish Shettigar, Archana Bulbule
<a href="http://www.accordinstitute.org/2009_5_2_impact_of_an_external_energy_on_enterococcus.htm" target="_blank" rel="noreferrer noopener">http://www.accordinstitute.org/2009_5_2_impact_of_an_external_energy_on_enterococcus.htm</a>
Accord Institute
2009
English
Journal Article
Impact of an external energy on Staphylococcus epidermis [ATCC –13518] in relation to antibiotic susceptibility and biochemical reactions – An experimental study
Microbiology
<p style="text-align:justify;">Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists. Results The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.</p>
Mahendra kumar Trivedi, Shrikant Patil
<a href="http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm" target="_blank" rel="noreferrer noopener">http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm</a>
Accord Institute
Nov. 2008
English
Journal Article
Impact of an external energy on Yersinia enterocolitica [ATCC –23715] in relation to antibiotic susceptibility and biochemical reactions: An experimental study
Microbiology
<p style="text-align:justify;">Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.</p>
Mahendra kumar Trivedi, Shrikant Patil
<a href="http://ispub.com/IJAM/6/2/5464" target="_blank" rel="noreferrer noopener">http://ispub.com/IJAM/6/2/5464</a>
Internet Scientific Publications
2008
English
Journal Article
Molecular Analysis of Biofield Treated Eggplant and Watermelon Crops
Plant Genetics
<p style="text-align:justify;">Eggplant and watermelon, as one of the important vegetative crops have grown worldwide. The aim of the present study was to analyze the overall growth of the two inbreed crops varieties after the biofield energy treatment. The plots were selected for the study, and divided into two parts, control and treated. The control plots were left as untreated, while the treated plots were exposed with Mr. Trivedi’s biofield energy treatment. Both the crops were cultivated in different fields and were analyzed for the growth contributing parameters as compared with their respective control. To study the genetic variability in both plants after biofield energy treatment, DNA fingerprinting was performed using RAPD method. The eggplants were reported to have uniform colored, glossy, and greener leaves, which are bigger in size. The canopy of the eggplant was larger with early fruiting, while the fruits have uniform shape and the texture as compared with the control. However, the watermelon plants after the biofield treatment showed higher survival rate, with larger canopy, bright and dark green leaves compared with the untreated plants. The percentage of true polymorphism observed between control and treated samples of eggplant and watermelon seed samples were an average value of 18% and 17%, respectively. Overall, the data suggest that Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate, and can be utilized in better way as compared with the existing agricultural crop improvement techniques to improve the overall crop yield.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/molecular-analysis-of-biofield-treated-eggplant-and-watermelon-crops-2329-8863-1000208.php?aid=68428" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/molecular-analysis-of-biofield-treated-eggplant-and-watermelon-crops-2329-8863-1000208.php?aid=68428</a>
Omics Publishing Group
January 31, 2016
English
Journal Article
10.4172/2329-8863.1000208
Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd
Plant Genetics
<p style="text-align:justify;">Plants are known to have sense and can respond to touch, electric and magnetic field. The present study was designed on the sponge gourd (Luffa cylindrica) and bitter gourd (Momordica charantia) seeds with respect to biofield energy treatment. The seeds of each crop were divided into two groups, one was kept control, while the other group was subjected to Mr. Trivedi’ biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. To study the genetic variability after biofield energy treatment, both the seeds were analyzed for DNA fingerprinting using RAPD method. After germination, the plants of sponge gourd were reported to have uniform colored leaves and strong stem. The leaves and fruits of sponge gourd showed no infection, with anti-gravity properties during early stage of fruiting as compared with the control group. Similarly, treated bitter gourd showed uniform color of leaves, strong stem, with disease free fruits in biofield treated seeds as compared with the control. The true polymorphism (%) observed between control and treated samples of sponge and bottle gourd seed sample was an average value of 7.8% and 66% respectively. In conclusion, Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate that may by interacting with plant genome, which resulted in high yield of crops.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=218&doi=10.11648/j.ajaf.20150306.14" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=218&doi=10.11648/j.ajaf.20150306.14</a>
Science Publishing Group
December 21, 2015
English
Journal Article
10.11648/j.ajaf.20150306.14
Evaluation of Vegetative Growth Parameters in Biofield Treated Bottle Gourd (Lagenaria siceraria) and Okra (Abelmoschus esculentus)
Plant Genetics
<p style="text-align:justify;">The objective of the study was to assess the growth contributing characters of biofield treated bottle gourd (Lagenaria siceraria) and okra (Abelmoschus esculentus) seeds. The seeds of both crops were divided into two groups, one was kept aside and denoted as untreated, while the other group was subjected biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. Further the level of glutathione (GSH) in okra leaves, along with DNA fingerprinting in bottle gourd were analyzed using RAPD method. After germination, the plants of bottle gourd were reported to be strong and erect with better canopy as compared with the control. The vegetative growth of okra plants after biofield energy treatment was found to be stout with small canopy, strong steam, and more fruits per nodes, that contributed high yield as compared with the control. However, endogenous level of GSH in the leaves of okra was increased by 47.65% as compared to the untreated group, which may suggest an improved immunity of okra crops. Besides, the DNA fingerprinting data, showed polymorphism (42%) between treated and untreated samples of bottle gourd. The overall results suggest that the biofield energy treatment on bottle gourd and okra seeds, results an improved overall growth of plant and yield, which may enhance flowering and fruiting per plant. Study results conclude that the biofield energy treatment could be an alternate method to improve the crop yield in agricultural science.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=153&doi=10.11648/j.ijnfs.20150406.24" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=153&doi=10.11648/j.ijnfs.20150406.24</a>
Science Publishing Group
December 21, 2015
English
Journal Article
10.11648/j.ijnfs.20150406.24
Agronomic Characteristics, Growth Analysis, and Yield Response of Biofield Treated Mustard, Cowpea, Horse Gram, and Groundnuts
Plant Genetics
<p style="text-align:justify;">Mustard, cowpea, horse gram, and groundnuts are the seasonal pulse crops used as food and fodder in many regions of the world. In the present study, the impact of biofield energy treatment on mustard, cowpea, horse gram, and groundnuts were studied with respect to overall growth, yield, and its related yield attributes. Seeds of each crop was selected and divided in two groups, i.e. control and treated. The treated group of each seed crops was subjected to Mr. Trivedi biofield energy treatment, and were plotted in the separate fields. The plot with untreated seeds were provided with all the precautionary measures such as pesticides, fungicides and organic additives, while no such measures were taken in the plot with treated seeds. Both group of crops were further analyzed and compared for growth, yield, and yield attributes. Further, the effect of biofield treatment was also evaluated on horse gram using Random Amplified Polymorphic DNA (RAPD) analysis in order to determine their epidemiological relatedness and genetic characteristics. The results suggest that the percentage increase in yield was maximum in mustard (500%), followed by horse gram (105%), cow pea (52%), and groundnut (44%) as compared with their control. However, improved plant height, overall growth, yield of seeds, plants were free from any diseases and pest were observed in treated group as compared with its respective control. RAPD analysis using eight primers results in polymorphism and the percentage of true polymorphism observed between control and treated samples of horse gram seed sample with an average value of 53%. The overall results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on mustard, cowpea, horse gram, and groundnuts, which might be used as a better alternative approach to increase the yield of crop as compared with the synthetic chemicals.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=116&doi=10.11648/j.ijgg.20150306.13" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=116&doi=10.11648/j.ijgg.20150306.13</a>
Science Publishing Group
November 9, 2015
English
Journal Article
10.11648/j.ijgg.20150306.13
Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (Gossypium hirsutum L.)
Plant Genetics
<p style="text-align:justify;">Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=218&doi=10.11648/j.ajaf.20150305.17" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=218&doi=10.11648/j.ajaf.20150305.17</a>
Science Publishing Group
November 9, 2015
English
Journal Article
10.11648/j.ajaf.20150305.17
Antimicrobial Susceptibility, Biochemical Characterization and Molecular Typing of Biofield Treated Klebsiella pneumoniae
Microbial Genetics
<p style="text-align:justify;">Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedi’s biofield energy treatment and analyzed for the antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotyping using automated MicroScan Walk-Away® system. Further, the effect of biofield treatment was also evaluated using Random Amplified Polymorphic DNA (RAPD) in order to determine their epidemiological relatedness and genetic characteristics of biofield treated K. pneumoniae samples. The antimicrobial susceptibility results showed an improve sensitivity (i.e. from intermediate to susceptible) of ampicillin/sulbactam and chloramphenicol, while altered sensitivity of cephalothin (i.e. from susceptible to intermediate) was also reported as compared to the control sample. The MIC value showed two-fold decrease in MIC value of ampicillin/sulbactam (i.e. 16/8 to ≤8/4 μg/mL) and chloramphenicol (i.e. 16 to ≤ 8 μg/mL) as compared to the control. The cephalothin showed two-folds change (i.e. ≤ 8 to 16 μg/mL) in the MIC value as compared with the control. Biofield treatment showed 9.09% alterations in biochemical reactions followed by a change in biotype number (7774 4272) in the treated group with respect to the control (7774 4274). Genetic fingerprinting was performed on control and treated samples using RAPD-PCR biomarkers, which showed an average range of 11 to 15% of polymorphism among the treated samples with respect to the control. These results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on K. pneumoniae.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/antimicrobial-susceptibility-biochemical-characterization-and-moleculartyping-of-biofield-treated-klebsiella-pneumoniae-2157-7420-1000206.php?aid=61143" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/antimicrobial-susceptibility-biochemical-characterization-and-moleculartyping-of-biofield-treated-klebsiella-pneumoniae-2157-7420-1000206.php?aid=61143</a>
Omics Publishing Group
September 25, 2015
English
Journal Article
10.4172/2157-7420.1000206