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https://d1y502jg6fpugt.cloudfront.net/25425/archive/files/4335b91e2dda3099940474832fc73f06.pdf?Expires=1712793600&Signature=Fcfh1kz1vRO15SS9oEwIIdXs2sFfW-wq9pGr-%7EhPVaJKTotRtdNf%7EU2UdMSehzoImdl9U49-I7aP-GI-rnxOj8HjJZThlYCQGE-F3tkm205PJm33zPjSgTF0bdcE9Myy-nqM4GCfPf8Z%7EKL-bvXwkOY7QIY0jp-zvn-w6asDOfQsuqPx1fJhS3CeFQZcr0nyLm9%7E51QCA9vs35zFm1S9S9krbL%7ELyB69JFbVqzndHZuvccXQeiiti20cF%7EmorcummiGZ2omKTW5zJwU7LUvJRBzD9eroW0E3IAfmTbPNjvl9V4ByGy38mnukRCRJTr2Z48kKBzGCGiFJ6jTygmsFoQ__&Key-Pair-Id=K6UGZS9ZTDSZM
1347180b82dcfd8636c1c7e0f2ce10ae
Website
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<a href="https://www.trivedieffect.com/science/lc-ms-gc-ms-and-nmr-spectroscopic-analysis-of-withania-somnifera-ashwagandha-root-extract-after-treatment-with-the-energy-of-consciousness-the-trivedi-effect">https://www.trivedieffect.com/science/lc-ms-gc-ms-and-nmr-spectroscopic-analysis-of-withania-somnifera-ashwagandha-root-extract-after-treatment-with-the-energy-of-consciousness-the-trivedi-effect</a>
Dublin Core
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Title
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LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)
Subject
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Nutraceuticals
Description
An account of the resource
<p style="text-align:justify;">Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-α, 6α-epoxy-β, 5β, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4β, 5β, 6α, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.</p>
Creator
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Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Parthasarathi Panda, Snehasis Jana
Source
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<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=117&doi=10.11648/j.ejb.20170502.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=117&doi=10.11648/j.ejb.20170502.11</a>
Publisher
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Science Publishing Group
Date
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March 09, 2017
Language
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English
Type
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Journal Article
Identifier
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10.11648/j.ejb.20170502.11
Biofield Energy Healers
Biofield Energy Healing Treatment
Consciousness Energy Healing Treatment
GC-MS
LC-MS
NMR
Retention Time
The Trivedi Effect®
Withania Somnifera
Withanolides
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Website
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Local URL
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<a href="https://www.trivedieffect.com/science/metabolite-profiling-of-withania-somnifera-roots-hydroalcoholic-extract-using-lc-ms-gc-ms-and-nmr-spectroscopy">https://www.trivedieffect.com/science/metabolite-profiling-of-withania-somnifera-roots-hydroalcoholic-extract-using-lc-ms-gc-ms-and-nmr-spectroscopy</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Metabolite Profiling in Withania somnifera Roots Hydroalcoholic Extract Using LC/MS, GC/MS and NMR Spectroscopy
Subject
The topic of the resource
Nutraceuticals
Description
An account of the resource
<p style="text-align:justify;">Ashwagandha (Withania somnifera) is a very well‐known herbal medicine and it was well studied for its active metabolites throughout the World. Although, nearly 40 withanolides were isolated from W. somnifera root extract, still there is remaining unidentified metabolites due to very low abundance and geographical variation. Advanced separation technology with online identification by mass and nuclear magnetic resonance (NMR) are nowadays used to find out the new compounds in the crude herbal extract. This article described the metabolite profiling of ashwagandha root hydroalcoholic extract using ultra‐performance liquid chromatography coupled with a positive ion electrospray ionization tandem mass spectrometry through gas chromatography mass spectrometry (GC/MS) and NMR spectroscopy. A total of 43 possible withanolides was identified and proposed their structures based on the mass of molecular and fragment ions. GC/MS and NMR analysis indicated the presence of several known withanolides including withaferin A, withanolide D, withanoside IV or VI, withanolide sulfoxide, etc. To the best of our knowledge, dihydrowithanolide D at m/z 473 (tR 7.86 min) and ixocarpalactone A at m/z 505 (tR 8.43 min) were first time identified in the ashwagandha root hydroalcoholic extract. The current study that described the identification of withanolides with summarized literature review might be helpful for designing the experiment to identify of the new chemical constituents in Withania species.</p>
Creator
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Mahendra Kumar Trivedi, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana
Source
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<a href="http://onlinelibrary.wiley.com/doi/10.1002/cbdv.201600280/abstract" target="_blank" rel="noreferrer noopener">http://onlinelibrary.wiley.com/doi/10.1002/cbdv.201600280/abstract</a>
Language
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English
Publisher
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Online Wiley Library
Date
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October 15, 2016
Identifier
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10.1002/cbdv.201600280
Type
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Journal Article
Ashwagandha Root Extract
Retention Time
Withania Somnifera
Withanolides