1,2,3-Trimethoxybenzene (TMB) is one of the most versatile chemical used for the synthesis of several pharmaceuticals, dyes, polymers, organic compounds, etc. The stable isotope ratio analysis has increased attention day-by-days in several fields such as agricultural, food authenticity, biochemistry, medical research, etc. The current study was aimed to evaluate the effect of the biofield energy treatment on the isotopic abundance ratios of 13C/12C or 2H/1H or 17O/16O (PM+1/PM) and 18O/16O (PM+2/PM) in TMB using Gas chromatography - mass spectrometry (GC-MS) technique. TMB was divided into two parts - one part was denoted as control and another part was referred as biofield energy treated sample that was received through Mr. Trivediꞌs unique biofield energy (The Trivedi Effect®). The GC-MS of the biofield treated TMB was characterized at different time intervals considered as T1, T2, T3, and T4 to examine the impact of the biofield energy treatment on isotopic abundance ratio with respect to the time. The GC-MS spectra of the both control and biofield treated TMB exhibited the presence of molecular ion peak [M+] at m/z 168 (calculated 168.08 for C9H12O3) along with similar pattern of fragmentation. The relative peak intensities of the fragmented ions in the biofield treated TMB, particularly at T2 and T3 was altered from the control sample. The isotopic abundance ratio analysis in the biofield treated TMB exhibited that the isotopic abundance ratio of PM+1/PM in the biofield treated TMB at T2 and T3 was significantly enhanced by 128.13 and 117.99%, respectively with respect to the control sample. Consequently, the percentage change in isotopic abundance ratio of PM+2/PM was significantly increased in the biofield treated TMB at T2 and T3 by 125.93 and 116.67%, respectively as compared with the control TMB. The isotopic abundance ratios (PM+1/PM and PM+2/PM) in the biofield treated TMB at T1 and T4 was altered with respect to the control TMB. In summary, 13C, 2H, and 17O contributions from (C9H12O3)+ to m/z 169 and 18O contribution from (C9H12O3)+ to m/z 170 for the biofield treated TMB, particularly at T2 and T3 were significantly improved and biofield treated TMB might exhibit changed isotope effects as compared to the control sample. The biofield treated TMB might assist to develop new chemicals and pharmaceuticals through using its kinetic isotope effects like understanding the reaction mechanism, the enzymatic transition state and all aspects of enzyme mechanisms.
The objective of the current study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of PM+1/PM, PM+2/PM, PM+3/PM and PM+4/PM in p-DCB using gas chromatography-mass spectrometry (GC-MS). The p-DCB was divided into two parts - one part was control sample, and another part was considered as the treated sample which was subjected to biofield energy treatment (The Trivedi Effect®). T1, T2, T3, and T4 were referred the biofield treated p-DCB having analyzed at different time intervals. The GC-MS analysis of both the control and biofield treated p-DCB indicated the presence of the parent molecular ion peak at m/z 146 along with four major fragmentation peaks at m/z 111, 75, 55 and 50. The relative peak intensities of the fragmented ions in the biofield treated p-DCB were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of PM+1/PM at T1, T2, T3, and T4 (biofield energy treated p-DCB) was significantly increased by 10.87, 83.90, 225.16, and 241.15%, respectively as compared to the control sample. Consequently, the percentage change in the isotopic abundance ratio of PM+2/PM at T1, T2, and T3 (biofield energy treated p-DCB) was enhanced by 4.55, 9.49, and 1.80%, respectively as compared to the control sample. Beside these, another two isotopic molecular ion peaks at m/z 149 and 150 were found in the GS-MS spectra due to arise from the contributions of various combinations of 2H, 13C, and 37Cl. The isotopic abundance ratios of PM+3/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 15.14, 82.57, 192.43, and 218.31%, respectively as compared to the control sample. Similarly, the PM+4/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 13.80, 86.66, 186.13, and 204.29%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C), PM+2/PM (37Cl/35Cl), for PM+3/PM and PM+4/PM (the probable combinations of 2H/1H, 13C/12C, and 37Cl/35Cl) were significantly enhanced in the biofield energy treated p-DCB. The biofield treated p-DCB has shown improved isotopic abundance ratios that might have altered the physicochemical properties, thermal properties and rate of reaction. Biofield treated p-DCB might be useful in pharmaceutical and chemical industries as intermediates during the manufacturing of pharmaceuticals and chemicals by monitoring the rate of chemical reaction.
Methyl-2-napthylether (nerolin) is an organic compound and has the applications in pharmaceutical, and perfume industry. The stable isotope ratio analysis is increasing importance in various field of scientific research. The objective of the current study was to evaluate the effect of the biofield energy treatment on the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O) and PM+2/PM (18O/16O) in nerolin using the gas chromatography-mass spectrometry (GC-MS). The compound nerolin was divided into two parts - one part was control sample (untreated), and another part was considered as biofield energy treated sample which was received the biofield energy treatment through the unique biofield energy transmission process by Mr. Mahendra Kumar Trivedi (also known as The Trivedi Effect®). The biofield energy treated nerolin was analyzed at different time intervals and were represented as T1, T2, T3, and T4 in order to understand the effect of the biofield energy treatment on isotopic abundance ratio with respect to the time. From the GC-MS spectral analysis, the presence of the molecular ion peak C11H10O+ (m/z 158) along with major fragmented peaks C10H7O- (m/z 143), C10H8 (m/z 128), C9H7+ (m/z 115), C7H5+ (m/z 89), C5H3+ (m/z 63), C4H3+ (m/z 51), and C3H3+ (m/z 39) were observed in both control and biofield treated samples. Only, the relative peak intensities of the fragmented ions in the biofield treated nerolin was notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis of nerolin using GC-MS revealed that the isotopic abundance ratio of PM+1/PM in the biofield energy treated nerolin at T1, T2, T3, and T4 was increased by 2.38, 138.10, 13.10, and 32.14%, as compared to the control sample. Likewise, the isotopic abundance ratio of PM+2/PM at T1, T2, T3, and T4 was increased by 2.38, 138.10, 13.10, and 32.14%, respectively in the biofield treated nerolin as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O) and PM+2/PM (18O/16O) were significantly increased in the biofield energy treated sample as compared to the control sample with respect to the time. It is concluded that Mr. Trivedi’s biofield energy treatment has the significant impact on alteration in isotopic abundance of nerolin as compared to the control sample. The biofield treated nerolin might display different altered physicochemical properties and rate of reaction and could be an important intermediate for the production of pharmaceuticals, chemicals, and perfumes in the industry.
1-Chloro-3-nitrobenzene (3-CNB) is an aromatic halo-amine compound used as chemical intermediate for the production of several fine chemicals like pharmaceuticals, dyes, agricultural chemicals, etc. The stable isotope ratio analysis has drawn attention in numerous fields such as agricultural, food authenticity, biochemistry, etc. The objective of the current research was to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of PM+1/PM, PM+2/PM and PM+3/PM in 3-CNB using gas chromatography - mass spectrometry (GC-MS). The sample, 3-CNB was divided into two parts - one part was denoted as control and another part was referred as biofield energy treated sample that was treated with biofield energy (The Trivedi Effect®). T1, T2, T3, and T4 were represented to different time interval analysis of the biofield treated 3-CNB. The GC-MS spectra of the both control and biofield treated 3-CNB indicated the presence of molecular ion peak [M+] at m/z 157 (calculated 156.99 for C6H4ClNO2) along with same pattern of fragmentation. The relative intensities of the parent molecule and other fragmented ions of the biofield treated 3-CNB were improved as compared to the control 3-CNB. The percentage change of the isotopic abundance ratio of PM+1/PM was significantly increased in the biofield treated 3-CNB at T1, T2 and T3 by 11.62, 18.50, and 29.82%, respectively with respect to the control 3-CNB. Accordingly, the isotopic abundance ratio of PM+2/PM in the biofield treated 3-CNB at T2 and T3 was significantly improved by 15.22 and 35.09%, respectively as compared to the control sample. The isotopic abundance ratios of PM+1/PM and PM+2/PM in the biofield treated 3-CNB at T1 and T4 were changed as compared to the control sample. The percentage change of the isotopic abundance ratio of PM+3/PM was enhanced in the biofield treated 3-CNB at T1, T2, T3, and T4 by 4.67, 18.69, 31.31 and 6.08%, respectively as compared to the control 3-CNB. The isotopic abundance ratios of PM+1/PM, PM+2/PM and PM+3/PM in the biofield treated 3-CNB changed with the time. So, the biofield energy treated 3-CNB might exhibit the altered isotope effects such as altered physicochemical and thermal properties, binding energy, and the rate of the chemical reaction as compared to the control sample. The biofield energy treated 3-CNB might assist in designing for the synthesis of pharmaceuticals, agricultural chemicals, dyes, corrosion inhibitors and other several useful industrial chemicals.
The objective of the current experiment was to evaluate the effect of biofield energy treatment on the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N) in indole using the gas chromatography-mass spectrometry (GC-MS). The sample of organic compound indole was divided into two parts - one part was designated as a control sample (untreated), and another part was considered as biofield energy treated sample, which was subjected to Mr. Trivedi’s biofield energy treatment (The Trivedi Effect®). The biofield energy treated indole sample was analyzed at different time intervals and were symbolized as T1, T2, T3, and T4 to understand the effect of the biofield energy on isotopic abundance ratio with respect to the time. From the GC-MS spectra, the presence of the molecular ion peak C8H7N+ (m/z 117) along with major fragmented peaks C7H6+ (m/z 90), C7H5+ (m/z 89), C5H3+ (m/z 63), C4H2+ (m/z 50), C3H3+ (m/z 39), and C2H4 (m/z 28) were observed in both control and biofield treated samples. Only, the relative peak intensities of the fragmented ions in the biofield treated indole was notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis of indole using GC-MS revealed that the isotopic abundance ratio of PM+1/PM in the biofield energy treated indole at T1 and T2 was significantly decreased by 44.28 and 28.18% as compared to the control sample. On the contrary, the isotopic abundance ratio of PM+1/PM in the biofield energy treated sample at T3 and T4, was significantly increased by 41.22 and 180.88%, respectively as compared to the control sample. Overall, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N) was significantly altered in the biofield energy treated indole as compared to the control with respect to the time. The biofield treated indole with the altered isotopic abundance ratio might have altered the physicochemical properties and rate of reaction. This biofield energy treated indole might be more useful as a chemical intermediate in the production of pharmaceuticals, chemicals, plastics, dyes, and perfumes.
The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the formulation when applied to splenocyte cells isolated from mice spleen. The formulation was divided into two parts, one was the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The test formulation was evaluated to find the expression of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β along with non-cytotoxic concentrations by MTT assay. The splenocytes were given the Biofield Energy Treated and untreated sample at concentrations range (0.00001053 to 10.53 µg/mL) for 48 hours and was reported with safe concentration up to 1.053 µg/mL with percentage viability range from 76.7% to 109.2% in both samples. Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated formulation. The expression of TNF-α was significantly inhibited in the Biofield Treated formulation at 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively compared with the untreated formulation. The rest of the tested concentrations of the Biofield Treated formulation showed an increase in TNF-α expression at 0.00001053, 0.0001053, and 0.001053 µg/mL by 7.26%, 8.50%, and 8.50%, respectively compared to the vehicle control group. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppression activity at 0.01053 µg/mL by 18.47% (p≤0.001) compared to the untreated formulation. MIP-1α expression was reported as 628.94 ± 13.0 pg/mL in the untreated formulation, while it decreased to 512.74 ± 1.9 pg/mL in the Biofield Treated formulation at 0.01053 µg/mL. In addition, the IL-1β secretion was also significantly inhibited by the Biofield Treated formulation at concentrations 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. Overall, The Trivedi Effect® significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the treated formulation, which can be better utilized in organ transplants, anti-aging, stress management, autoimmune disorders, and inflammatory disorders, etc. to modulate the immune system by improving overall health.
The use of herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal root extract (ashwagandha), and minerals (zinc, magnesium, and selenium). The study aims to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine dendritic (DCs) and splenocyte cells. The herbomineral formulation was divided into two parts, one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The effect of the test formulation on these cells were monitored by an estimation of pro-inflammatory cytokines level such as tumor necrosis factor (TNF-α), macrophage inflammatory protein (MIP-1α), and interleukin (IL-1β) in cell culture supernatants at the non-cytotoxic concentrations of the test formulation using MTT assay. The DCs were treated with the Biofield Energy Treated test formulation at different concentrations (i.e. 1.05 to 1052.5 µg/mL) for 24 hours, and the results showed significant (p≤0.001) suppression of TNF-α levels at all the tested concentrations with a maximum percentage decrease by 43.64% at 5.2 µg/mL concentration in the Biofield Treated formulation as compared with the untreated test formulation. Further, the Biofield Treated formulation also demonstrated inhibition of MIP-1α and IL-1β at a concentration range of 0.0000105 to 10.5 µg/mL in LPS stimulated splenocyte cells. There was a significant (p≤0.001) inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated test formulation at concentration 0.000105 µg/mL and 0.0000105 µg/mL, respectively in comparison to the untreated test formulation. Overall, these results suggest that the Biofield Energy Healing based herbomineral formulation (The Trivedi Effect®) significantly down-regulated the tested cytokines expression in DCs and splenocyte cells as compared to the untreated formulation. Therefore, the Biofield Healing based formulation might be useful as a better anti-inflammatory product for many chronic and acute inflammatory disease conditions and autoimmune disorders. The Biofield Energy Treatment based formulation can also be effectively applied in cases of organ transplants, stress management and anti-aging by improving overall health.
The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
Due to the increased popularity of herbomineral preparations in the healthcare sector, a new proprietary herbomineral formulation was formulated consisting of ashwagandha root extract and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells. The herbomineral formulation was divided into two parts, one defined as the control, while the other part was treated with the Biofield Energy Healing Treatment performed from a remote distance by twenty renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The splenocyte cells were exposed to test formulations at concentration from 0.00001053 to 10.53 µg/mL and were analyzed after 48 hours for cell viability using MTT assay. The expression of the cytokines (TNF-α, IFN-γ, IL-1β, and MIP-1α) was determined using ELISA assay. The cell viability data showed that all the tested concentration ranges were found to be safe with percentage cell viability at more than 80%. Further, TNF-α expression was significantly inhibited in the Biofield Treated test formulation group with respect to the vehicle control, while at 0.001053 and 0.1053 µg/mL, the expression was suppressed by 1.70% and 8.16%, respectively in the Biofield Treated test formulation compared to the untreated formulation. However, a significant immunosuppression was reported in IFN-γ expression at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The MIP-1α expression was inhibited by 8.31%, 21.53%, and 8.70% at 0.0001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated formulation compared with the untreated test formulation. However, IL-1β expression was significantly suppressed by 19.72% at concentration 0.00001053 µg/mL in the Biofield Treated test formulation compared with the untreated test formulation. Thus, the down-regulation of tested cytokines and chemokines in the Biofield Energy Healing test formulation might be applicable for controlling acute and chronic inflammation in many clinical diseases. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory activity of the test formulation, which can be useful against autoimmune disorders. Biofield Treated Test formulation may also be useful in anti-aging, anti-inflammatory, stress management and in preventing immune-mediated tissue damage in organ transplants by improving overall health and quality of life.
Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation when applied to splenocyte cells isolated from the Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment. The other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. A wide concentration range (0.00001053 to 10.53 µg/mL) of the test formulation was used to determine non-cytotoxic concentrations using MTT assay. Further, the expression of pro-inflammatory cytokines (TNF-α, MIP-1α, and IL-1β) was determined by ELISA method. The test formulation was evaluated and found to be safe up to 1.053 µg/mL with a percentage cell viability range of 73% to 97% using MTT assay. The Biofield Treated formulation improved the cell viability up to 6.61% compared with the untreated test formulation. TNF-α expression was significantly inhibited by 16.72% at 0.1053 µg/mL compared with the untreated test formulation, however expression was significantly altered by 53.67% and 25.62% at 0.01053 and 1.053 µg/mL, respectively compared to the untreated test formulation. TNF-α expression was also suppressed in the Biofield Treated test formulation at 0.001053 and 0.1053 µg/mL by 4.0% and 8.56%, respectively as compared with the vehicle control. MIP-1α suppression was reported in the Biofield Treated test formulation at 0.00001053 to 1.053 µg/mL by 8.43%, 22.02%, 21.92%, 20.54%, 5.40%, and 19.82%, respectively compared with the vehicle control. However, the Biofield Treated formulation further exhibited substantial suppression of MIP-1α at 0.0001053, 0.001053, 0.01053, and 0.1053 µg/mL by 13.50%, 7.38%, 36.83% (p≤0.001), and 2.53%, respectively compared with the untreated test formulation. In addition, significant inhibition of IL-1β secretion was reported in the Biofield Treated formulation at 0.0001053, 0.001053, 0.01053, and 0.01053 µg/mL by 32.40%, 14.99%, 60.42%, and 15.15%, respectively compared with the untreated test formulation. The Biofield Energy Healing Treatment significantly potentiated the immunosuppressive effect of the test formulation in Biofield Treated mouse splenocytes, which can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.