Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Ashwagandha root extract is useful as an herbal medicine and nutraceuticals for the prevention and treatment of various diseases. The aim of the current study was to evaluate the influence of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the physico-chemical, thermal and behavioral properties of ashwagandha root extract using powder X-ray diffraction (PXRD), particle size distribution analysis (PSD), Fourier transform infrared (FT-IR) spectrometry, UV-Vis spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment, and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis concluded that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were significantly decreased by 36.78%, 15.18%, and 5.06%, respectively compared with the control sample. Likewise, the surface area of the treated sample was significantly increased by 85.14% compared to the control sample. FT-IR results showed a small impact of Consciousness Energy Healing Treatment on the phytoconstituents of ashwagandha root extract to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance (λmax) of both the samples was at 206.4 in methanol. TGA revealed the three steps of thermal degradation and the total weight loss was decreased by 0.73% in the treated sample compared to the control sample. Consequently, the maximum thermal degradation temperature was found at 272.53°C and 393.35°C for two broad peaks in the treated sample was increased by 0.05% and 0.08%, respectively compared to the control the sample (272.67°C and 393.66°C). The DSC analysis indicated that the evaporation temperature and latent heat of vaporization were lowered significantly by 4.98% and 35.67%, respectively in the treated sample compared with the control sample. The current outcomes suggested that the Energy of Consciousness Healing Treatment might have the amazing capacity to enhance the solubility, dissolution, absorption, bioavailability and thermal stability of ashwagandha root extract in the various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the Biofield Energy Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, ageing, diabetes, and other chronic infections.
Withania somnifera (Ashwagandha) is useful as an herbal medicine and nutraceuticals for the prevention and treatment of anxiety, insomnia, tuberculosis, tumors, menstrual problems, etc. The objective of the current study was to evaluate the influence of The Trivedi Effect® - Consciousness Energy Healing Treatment on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis demonstrated that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were decreased significantly by 18.88%, 8.60%, and 4.00%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly increased by 102.63% compared to the control sample. FT-IR results suggest that there was a small impact of Biofield Energy Treatment on ashwagandha root extract at the atomic level to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.4 and 207.7 nm, respectively. TGA revealed the three steps of thermal degradation steps and the total weight loss was decreased by 0.13% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 272.73°C and 393.14°C for two broad peaks in treated sample was increased by 1.13% and 0.85%, respectively compared to the control the sample (269.68°C and 389.83°C). DSC analysis indicated that the evaporation temperature and latent heat of vaporization was increased by 1.35% and 5.52%, respectively in the treated sample compared with the control sample. The current findings suggested that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, absorption, dissolution, bioavailability and thermal stability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, sexual disorders, stress, cancer, diabetes, ageing, and other chronic infections.
Withania somnifera (Ashwagandha) root extract has a broad range of pharmacological activities due to the presence of many biologically active metabolites. The current study was designed to evaluate the influence of The Trivedi Effect® - Consciousness Energy Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis exhibited that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were increased significantly by 8.69%, 7.32%, and 6.50%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly decreased by 12.69% compared to the control sample. The FT-IR analysis indicated no significant alteration of the force constant for the functional groups in the treated sample compared to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.5 and 205.2 nm, respectively. The TGA revealed the two major thermal degradation steps and the total weight loss was decreased by 0.86% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 273.53°C and 389.39°C for two broad peaks in treated sample was increased by 0.78% and 0.31%, respectively compared to the control the sample (271.40°C and 388.48°C). The DSC analysis indicated that the evaporation temperature was significantly increased by 10.95% in the Biofield Energy Treated sample with a 5.34% reduced the latent heat of vaporization compared with the control sample. The overall thermal analysis indicated that the treated sample was thermally more stable as compared to the control sample. The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to have better powder flowability and long-term storage stability compared with the control sample. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, ageing, and other chronic infections.
Ashwagandha (Withania somnifera) is a well-known traditional medicinal herb and its root extract have a broad range of pharmacological activities. The current study aimed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physicochemical, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle size distribution values of the treated sample at d10, and d50 were increased by 16.94% and 8.01%, respectively, whereas at d90 particle size value was decreased by 7.02% compared with the control sample. The surface area analysis revealed that the surface area of the treated sample was decreased by 4.17% compared with the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample compared with the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.2 nm. The TGA analysis revealed that the total weight loss was decreased by 1.13% in the treated sample as compared to the control sample. Similarly, the DTG analysis revealed that the maximum thermal degradation temperature in the treated sample was increased by 0.14% compared to the control the sample. The DSC analysis indicated that the vaporization temperature and the latent heat of vaporization of the treated sample were increased by 1.45% and 11.30%, respectively as compared to the control sample. Similarly, onset, peak, endset melting temperature and the latent heat of fusion of the treated sample were significantly increased by 18.90%, 18.86%, 20.18%, and 511.11%, respectively compared to the control sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to have better powder flowability and long-term storage stability compared with the control sample. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Withania somnifera (Ashwagandha) root extract contains lot of biologically active metabolites, which have a broad range of pharmacological activities. The current study was designed to evaluate the effects of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were decreased by 8.41%, 0.51%, and 7.88%, respectively compared with the control sample. The surface area analysis revealed that the surface area of the treated sample was significantly increased by 5% compared to the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample in comparison to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.3 and 205.0 nm, respectively. The TGA analysis revealed that the total weight loss was decreased by 0.65% in the treated sample compared with the control sample. The DSC analysis indicated that the onset, peak, and endset vaporization temperature of the treated sample were significantly increased by 41.97%, 23.51%, and 8.82%, respectively as compared to the control sample. The latent heat of vaporization (∆H) of the treated (239.96 J/g) sample was significantly increased by 39.84% compared with the control (171.60 J/g) sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The current findings suggested that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) might have the astounding capacity to enhance the solubility, absorption, dissolution, and bioavailability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect® treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of - 4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4-dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy-γ-butyrolactone (3) was decreased by - 14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect® - Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.
Withania somnifera (Ashwagandha) root extract possesses a broad range of pharmacological activities. The aim of current study was to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis exhibited that both the control and The Trivedi Effect® - Biofield Energy Treated samples were amorphous in nature. The particle size values at d10 and d50 of the treated sample were decreased by 4.66% and 6.02% and at d90 slightly increased by 0.99% compared with the control sample. The average surface area of the treated (0.130 m2/g) sample was increased by 4.84% compared with the control (0.124 m2/g) sample. The FT-IR results suggested that the force constant of O-H (stretching), and C-O (stretching) functional groups of the treated sample were reduced compared with the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.4 nm and 205.6 nm, respectively. The TGA thermograms exhibited three steps of the degradation process, and the total weight loss of the treated (86.45%) sample was increased by 1.44% compared with the control (85.22%) sample. The DSC analysis revealed that melting point of the treated (201.67°C) sample was reduced by 0.87% compared with the control (203.43°C) sample. The latent heat of fusion of the treated (6.77 J/g) sample was significantly decreased by 21.82% compared to the control (8.67 J/g) sample. Hence, the treated sample would be more advantageous for the better therapeutic responses compared to the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, dissolution, absorption, and bioavailability of ashwagandha root extract in various forms of pharmaceutical/nutraceutical formulations by modifying its particle size and surface area. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-α, 6α-epoxy-β, 5β, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4β, 5β, 6α, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.