Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract
Nutraceuticals
<p style="text-align:justify;">Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=117&doi=10.11648/j.ejb.20170502.12" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=117&doi=10.11648/j.ejb.20170502.12</a>
Science Publishing Group
May 08, 2017
English
Journal Article
10.11648/j.ejb.20170502.12
Physicochemical, Thermal and Spectroscopic Characterization of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract
Material Science
"Ashwagandha root extract is widely used in nutraceutical preparations due to its broad range of pharmacological activities.
The objective of this study was to analyse the impact of Biofield Energy Treatment (The Trivedi Effect®) on the physicochemical,
thermal and spectral properties of ashwagandha root extract by using various analytical techniques. The sample of
ashwagandha root extract was divided into two parts in which one part was kept untreated and called as control sample; while
the other part was provided with the Consciousness Energy Healing Treatment remotely by the Biofield Energy Healer, Mr.
Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The study reported that the particle size values at d10,
d50, d90, and D (4,3) in the Biofield Energy Treated sample was significantly decreased by 9.23%, 9.04%, 11.86%, and 10.59%,
respectively compared with the control sample. Thus, the surface area of the treated sample was significantly increased by
10.75% compared to the control sample. The PXRD analysis showed that the control as well as the Biofield Energy Treated
samples was amorphous in nature. The DSC analysis showed that the onset evaporation temperature and latent heat of
vaporization were significantly altered by 2.18% and-10.29%, respectively in the Biofield Energy Treated sample compared
with the control sample. The TGA analysis indicated the four step thermal degradation of both the samples. The 1st, 2nd, 3rd,
and 4th steps of thermal degradation of the treated sample showed an alteration in the weight loss by -6.59%, 1.97%, -1.80%,
and -8.65%, respectively along with 0.81% reduction in the total weight loss, compared with the control sample. Besides, the
maximum thermal degradation temperature (Tmax) in the control sample was observed at 234.17°C and 365.12°C for the two
broad peaks, which was decreased by 1.73% and 0.82%, respectively compared to the control sample. The overall analysis
suggests that the Energy of Consciousness Healing Treatment might enhance the solubility, absorption, and bioavailability
profile of ashwagandha root extract along with altered thermal stability. Such altered properties might help in designing better
pharmaceutical and nutraceutical preparations; thereby provide better therapeutic response against various diseases such as
amnesia, arthritis, anxiety, cancer, impotence, neurodegenerative, and cardiovascular diseases."
Mahendra Kumar Trivedi and Snehasis Jana
<a href="https://medwinpublishers.com/JONAM/volume.php?volumeId=350&issueId=1092">https://medwinpublishers.com/JONAM/volume.php?volumeId=350&issueId=1092</a>
Medwin
28-Jan-2021
English
Journal Article