Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution
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Title
Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution
Subject
Materials Science, Minerals
Description
Magnesium gluconate is a classical pharmaceutical compound used as a source of magnesium for the prevention and treatment of hypomagnesemia. To the best of our knowledge, a robust and reliable liquid chromatography tandem mass spectrometry technique has not yet been reported for the qualitative and quantitative analysis of magnesium gluconate. This study describes the method development for the LC–ESI–MS/MS analysis of magnesium gluconate using three different reversed-phase HPLC conditions (Method I–III) with comprehensive fragmentation pattern and the structural characterization by NMR spectroscopy. The LC–MS and NMR data were found in accordance with the structure of magnesium gluconate. When magnesium gluconate was dissolved in the acetonitrile and water–methanol solutions, it exists in situ in three different forms: magnesium gluconate itself, gluconic acid, and magnesium gluconate chelate with gluconic acid by a coordinate covalent bond. Method I exhibited pseudo-molecular ion peaks with more magnesium gluconate chelates with gluconic acid, while method II showed an adduct of magnesium gluconate with the solvent along with the molecular ion peak. There was no pseudo-molecular ion peaks found in method III. Thus, method III was found to be the more accurate, robust and reliable LC–MS method for the qualitative and quantitative analysis, structural characterization, and could also be suitable for the pharmacokinetic study of magnesium gluconate. The detailed fragmentation analysis might be useful for the structural characterization of unknown divalent organometallics.
Creator
Mahendra Kumar Trivedi, Parthasarathi Panda, Kalyan Kumar Sethi and Snehasis Jana
Publisher
Springer
Date
April 13, 2017
Contributor
[no text]
Rights
[no text]
Relation
[no text]
Format
[no text]
Language
English
Type
Journal Article
Identifier
10.1007/s10953-017-0613-z
Coverage
[no text]
Files
Citation
Mahendra Kumar Trivedi, Parthasarathi Panda, Kalyan Kumar Sethi and Snehasis Jana
, “Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution,” Mahendra Trivedi, accessed October 14, 2024, https://mahendratrivedi.omeka.net/items/show/265.