Immunomodulatory Action of a Proprietary Product Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

Immunomodulatory Action of a Proprietary Product Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

Health & Wellness

"Immunomodulatory activity of the novel formulation with respect to gut health using TNBS induced ulcerative colitis was studied in the present experiment. The action of Biofield Energy Healing (the Trivedi Effect®) based test formulation and Biofield Energy Treatment per se was studies. The test formulation consisted of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3). Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Additionally, three group of animals also received Biofield Energy Treatment per se (day -15) under similar conditions. The immune biomarkers such as immunoglobulin M (IgM), IgG, IgA, IgE, biochemistry, and hematology parameters were evaluated. The level of IgE was significantly increased by 22.95%, 25.51%, and 48.57% (p≤0.001) in the G5, G6, and G7 groups, respectively as compared with the untreated test group (G4). IgM level was significantly (p≤0.001) increased by 12.16%, 20.27%, and 11.49% in the G7, G8, and G9 groups, respectively as compared with the G4 group. The level of IgG was significantly (p≤0.001) increased by 11.19% in the G9 group as compared with the G4 group. Hematology data suggested significant increased the levels of total leukocyte count (TLC) (25.95%; p≤0.05 in G9), neutrophils (65.26%, 59.62%, and 117.84% (p≤0.05) in G6, G7, and G9 respectively), and monocytes (61.97% in G9) were reported when compared with the G4 group. Further, the level
of lymphocytes were significantly (p≤0.001) increased by 98.24%, 70.31%, 61.91%, and 108.20% in the G5, G6, G8, and G9 groups, respectively as compared to the disease control (G2) group. Serum glutamic-oxaloacetic transaminase (SGOT) or AST level was significantly reduced by 31.96% (p≤0.05), 23.24%, 23.44%, 24.45%, and 23.93% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Moreover, serum glutamic pyruvic transaminase (SGPT) or ALT level was reduced by 22.63% and 23.44% in G6 group as compared to G2 and G6 groups, respectively. The level of alkaline phosphatase (ALP) was reduced by 28.42%, 21.50%, 17.91%, 17.00%, and 21.63% in the G5, G6, G7, G8, and G9 groups, respectively than G2 group. Creatine kinase-myocardial band (CK-MB) level was significantly (p≤0.001) decreased by 46.26%, 28.88%, 42.33%, 43.57%, and 43.97%, respectively in G5, G6, G7, G8, and G9 groups, respectively as compared with the G4 group. The level of testosterone was increased by 45.96% and 35.31% in the G6 group as compared with the G2 and G4 groups, respectively. In addition, animal body weight, feed intake, and relative organ weight data did not showed any abnormal findings with respect to the safe and non-toxic treatment strategies. In conclusion, the Trivedi Effect®-Biofield Energy Healing Treatment and the test formulation has the significant capacity for immunomodulatory effect, which supposed to be found effective against overall gut health and inflammation."

Trivedi MK and Jana S

Academic Strive

18-Mar-2021

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English

Journal Article

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