Investigation of Cardiac Biomarkers in Aorta after Treatment with the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
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Title
Investigation of Cardiac Biomarkers in Aorta after Treatment with the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats
Subject
Health & Wellness
Description
"The aim of this study was to evaluate the effect of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on cardiac biomarkers in aorta homogenate on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. The functional aortic biomarkers such as inducible nitric oxide synthase (iNOS), angiotensin-II, C-reactive protein (CRP), cholesterol, troponin-1, and Na+/K+-ATPase were measured using standard ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic
acid, pyridoxine HCl, vitamin B9, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. In this experiment, nine groups were assigned, in which four were preventive maintenance groups. The ingredients were used in this formulation divided into two parts; one was defined as the unblessed test formulation, while the other portion of the test formulation received Biofield Energy Healing Treatment/Blessing remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Among nine groups, three groups of animals were also received Blessing per se (at day -15). The results showed that the level of iNOS was significantly (p≤0.001) reduced by 32.4%, 27.8%, and 19.8% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), and G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the disease control group (G2). Moreover, the level of angiotensin-II was significantly decreased by 33.6%, 47.2% (p≤0.001), 27.9%, 25.4%, and 22.3% in the G5, G6, G7, G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the G2 group. The level of CRP was decreased by 26.1%, 25.7%, 15.8%, and 16.6% in the G5, G6, G7, and G9 groups, respectively as compared to the G2 group. Besides, the level of cholesterol was significantly (p≤0.001) decreased by 62.4%, 54.5%, 52%, 46.2%, and 64.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of troponin-1 was significantly (p≤0.001) decreased by 42.8%, 42.5%, 36.7%, 28.9%, and 21.2% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of Na+/K+-ATPase was decreased by 14.8% in the G8 group as compared to the G4 group. Overall, the data suggested significance improvement of vital functional aortic biomarkers of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the cardiovascular diseases and its complications in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated/Blessed Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group."
acid, pyridoxine HCl, vitamin B9, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. In this experiment, nine groups were assigned, in which four were preventive maintenance groups. The ingredients were used in this formulation divided into two parts; one was defined as the unblessed test formulation, while the other portion of the test formulation received Biofield Energy Healing Treatment/Blessing remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Among nine groups, three groups of animals were also received Blessing per se (at day -15). The results showed that the level of iNOS was significantly (p≤0.001) reduced by 32.4%, 27.8%, and 19.8% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), and G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the disease control group (G2). Moreover, the level of angiotensin-II was significantly decreased by 33.6%, 47.2% (p≤0.001), 27.9%, 25.4%, and 22.3% in the G5, G6, G7, G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the G2 group. The level of CRP was decreased by 26.1%, 25.7%, 15.8%, and 16.6% in the G5, G6, G7, and G9 groups, respectively as compared to the G2 group. Besides, the level of cholesterol was significantly (p≤0.001) decreased by 62.4%, 54.5%, 52%, 46.2%, and 64.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of troponin-1 was significantly (p≤0.001) decreased by 42.8%, 42.5%, 36.7%, 28.9%, and 21.2% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of Na+/K+-ATPase was decreased by 14.8% in the G8 group as compared to the G4 group. Overall, the data suggested significance improvement of vital functional aortic biomarkers of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the cardiovascular diseases and its complications in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated/Blessed Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group."
Creator
Trivedi MK, Branton A, Trivedi D and Jana S
Publisher
Academic Strive
Date
23-Jun-2021
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Language
English
Type
Journal Article
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Citation
Trivedi MK, Branton A, Trivedi D and Jana S
, “Investigation of Cardiac Biomarkers in Aorta after Treatment with the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats,” Mahendra Trivedi, accessed May 1, 2024, https://mahendratrivedi.omeka.net/items/show/505.