An Alternative Approach for the Management of Bone Health: Role of Biofield Energy Healing Treated Vitamin D3
Nutraceuticals
<p style="text-align:justify;">The main aim of this experiment was to assess the impact of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health parameters. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Brian Anthony Weekes and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Different parameters like ALP, collagen, and bone mineralization were performed to evaluate the bone strength and integrity in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT cell viability assay. ALP was significantly increased by 19.49% and 63.49% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 1 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 35.92% and 61.17% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 53.75% and 42.43% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 1 µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 46.54% and 122.61% at 50 and 100 µg/mL, respectively in the BT-DMEM + UT-Test item group compared to the untreated group. Additionally, the percent of bone mineralization was distinctly increased by 18.12%, 96.33%, and 150.85% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the untreated group. Further, the percentage of bone mineralization was significantly increased by 61.96 and 22.84% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease of bone, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.</p>
Brian Anthony Weekes, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=252&doi=10.11648/j.ajim.20180601.11">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=252&doi=10.11648/j.ajim.20180601.11</a>
Science Publishing Group
10 January 2018
English
Journal Article
10.11648/j.ajim.20180601.11
An Alternative Therapeutic Approach on Bone Cells Differentiation after Administration of Biofield Energy Treated Vitamin D3 in MG-63 Cell line
Nutraceuticals
<p style="text-align:justify;">The objective of the current experiment was to evaluate the potential of Consciousness Energy Healing-based test items (Vitamin D3 and DMEM) on bone parameters. Both the TI were distributed into two individual parts. One part of each samples was received Consciousness Energy Healing Treatment by Eileen Mary Meagher and those samples were labelled as the Biofield Treated (BT) samples, while the other parts of each samples were denoted as the untreated test items (UT). Cell viability data showed more than 71% viable cells signifies a safe and nontoxic profile of test items on MG-63 cell line. ALP was significantly increased by 132.18% and 126.52% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 50 µg/mL; while 139.06% (at 0.1 µg/mL) in UT-DMEM+BT-TI compared to untreated. Collagen was significantly increased by 172.76% at 1 µg/mL in UT-DMEM+BT-TI, while 89.65% in BT-DMEM+UT-TI at 10 µg/mL than untreated. Additionally, collagen was significantly increased by 111.2% in BT-DMEM+UT-TI at 50 µg/mL, while 113.16% in BT-DMEM+BT-TI at 100 µg/mL than untreated. The percent of bone mineralization was significantly increased by 206.21%, 168.74%, and 224.82% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared with the untreated. In addition, percent of bone mineralization data showed a significant increased by 190.63%, 181.07%, and 245.41% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 1 µg/mL than untreated. Moreover, at 10 µg/mL it was significantly increased by 129.44%, 111.76%, and 109.81% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 10 µg/mL with respect to untreated. Based on the study outcomes it emphasized that Biofield Energy Treatment could regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases.</p>
Eileen Mary Meagher, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
<a href="http://www.avensonline.org/fulltextarticles/JPP-2327-204X-06-0023.html">http://www.avensonline.org/fulltextarticles/JPP-2327-204X-06-0023.html</a>
Avens Publishing Group
05 July 2018
English
Journal Article
An Effect of Biofield Treatment on Multidrug-resistant Burkholderia Cepacia: A Multihost Pathogen
Microbiology
<p style="text-align:justify;">Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10 days of treatment and compared with control group. Control and treated group were analyzed for susceptibility pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system. Sensitivity assay results showed a change in pattern from resistant to intermediate in aztreonam, intermediate to resistant in ceftazidime, ciprofloxacin, imipenem, and levofloxacin while sensitive to resistant in meropenem and piperacillin/tazobactam. The biofield treatment showed an alteration in MIC values of aztreonam, ceftazidime, chloramphenicol, ciprofloxacin, imipenem, levofloxacin, meropenem, piperacillin/tazobactam and tetracycline. Biochemical reactions of treated group showed negative reaction in colistin, lysine, and ornithine while positive reactions to acetamide, arginine, and malonate as compared to control. Overall results showed an alteration of 38.9% in susceptibility pattern, 30% in MIC values of tested antimicrobials and 18.2% change in biochemical reaction after biofield treatment. A significant change in biotype number (02063736) was reported with green pigment as special characteristics after biofield treatment as compared to control (05041776) group with yellow pigment. In treated group, a new species was identified as Pseudomonas aeruginosa, as compared to control. Study findings suggest that biofield treatment has a significant effect on the phenotypic character and biotype number of multidrug resistant strain of B. Cepacia.</p>
Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/an-effect-of-biofield-treatment-on-multidrugresistant-burkholderia-cepacia-a-multihost-pathogen-2329-891X-1000167.php?aid=56213" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/an-effect-of-biofield-treatment-on-multidrugresistant-burkholderia-cepacia-a-multihost-pathogen-2329-891X-1000167.php?aid=56213</a>
Omics Publishing Group
July 17, 2015
English
Journal Article
10.4172/2329-891X.1000167
An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen
Microbiology
<p style="text-align:justify;">Stenotrophomonas maltophilia (S. maltophilia) is a Gram-negative bacillus, an opportunistic pathogen, particularly among nosocomial infections. Multi-drug resistant strains are associated with very high rate of morbidity and mortality in severely immunocompromised patients. Present study was designed to evaluate the effect of biofield treatment against multidrug resistant S. maltophilia. Clinical sample of S. maltophilia was collected and divided into two groups i.e. control and biofield treated which were analyzed after 10 days with respect to control. The following parameters viz. susceptibility pattern, minimum inhibitory concentration (MIC), biochemical studies and biotype number of both control and treated samples were measured by MicroScan Walk-Away® system. The results showed an overall change of 37.5% in susceptibility pattern and 39.4% in biochemical study while 33.3% changes in MIC values of tested antimicrobials after biofield treatment. Further, the treated group of S. maltophilia has also shown a significant change in biochemical reactions followed by its biotype number as compared to control group. Biochemical reactions of treated group showed negative reaction to acetamide and positive reactions to colistin, glucose, adonitol, melibiose, arabinose, nitrate, oxidation-fermentation, raffinose, rhaminose, sorbitol, sucrose, and Voges-Proskauer as compared with control. The biofield treatment showed an alteration in MIC values of amikacin, amoxicillin/K-clavulanate, chloramphenicol, gatifloxacin, levofloxacin, moxifloxacin, ceftazidime, cefotetan, ticarcillin/K-clavulanate, trimethoprim/sulfamethoxazole. Altogether, data suggest that biofield treatment has significant effect to alter the sensitivity pattern of antimicrobials and biotype number against multidrug resistant strain of S. Maltophilia.</p>
Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/an-evaluation-of-biofield-treatment-on-susceptibility-pattern-of-multidrugresistant-stenotrophomonas-maltophilia-an-emerging-global-opportunisticpathogen-2327-5073-1000211.php?aid=57137" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/an-evaluation-of-biofield-treatment-on-susceptibility-pattern-of-multidrugresistant-stenotrophomonas-maltophilia-an-emerging-global-opportunisticpathogen-2327-5073-1000211.php?aid=57137</a>
Omics Publishing Group
July 24, 2015
English
Journal Article
10.4172/2327-5073.1000211
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder
Materials Science
<p style="text-align:justify;">Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated. The treated group received Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using X-ray diffraction (XRD), differential scanning calorimetry (DSC), particle size analyzer, surface area analyzer, and scanning electron microscopy (SEM). XRD results showed significant alteration in lattice parameter, unit cell volume, densities, nuclear charge per unit volume, and atomic weight in treated cadmium powder as compared to control. Furthermore, crystallite size was significantly reduced upto 66.69% in treated cadmium as compared to control. DSC analysis results showed that the latent heat of fusion of the treated cadmium powder was considerably reduced by 16.45% as compared to control. Particle size data revealed that average particle size (d50) of treated cadmium powder was significantly reduced by 47.79 % as compared to the control. In addition, the surface area of treated cadmium powder was substantially enhanced by 156.36% as compared to control. Surface morphology observed by SEM showed the more facets and fractured surface with satellite boundaries in treated cadmium powder as compared to control. These findings suggest that biofield treatment has significantly altered the atomic, thermal and physical properties of cadmium.</p>
Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/an-evaluation-of-biofield-treatment-on-thermal-physical-and-structuralproperties-of-cadmium-powder-2157-7544-1000147.php?aid=58357" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/an-evaluation-of-biofield-treatment-on-thermal-physical-and-structuralproperties-of-cadmium-powder-2157-7544-1000147.php?aid=58357</a>
Omics Publishing Group
August 04, 2015
English
Journal Article
10.4172/2157-7544.1000147
An Evaluation of Vitamin D Receptor (VDR), Telomerase and Citrate Synthase in Various Tissues after Treatment with the Biofield Energy Healing based Test Formulation in Vitamin D3 Deficiency Diet (VDD) Induced Animal Model
Health & Wellness
The study was evaluated the impact of Consciousness Energy Healing Treatment/Blessing (the Trivedi Effect®) on test formulation in rats under the influence of vitamin D3 deficiency diet (VDD). The following mechanistic biomarkers were estimated such as vitamin D receptor (VDR), telomerase, and citrate synthase enzymes. The test formulation’s ingredient was divided into two parts; one part was defined as untreated test formulation, while the other parts and the animals provided Biofield Energy Healing/Blessing Treatment by Mr. Mahendra Kumar Trivedi. The expression of VDR in liver was significant increased by 74.91%, 100.66%, and 64.25% in the G5 (VDD + Biofield Energy Treated test formulation), G6 (VDD + Biofield Energy Treatment per se to animals from day -15), and G7 (VDD + Biofield Energy Treated test formulation from day -15) groups, respectively as compared with the disease control (G2) group. Similarly, the expression of VDR in adipose tissue was significant increased by 268.6% (p≤0.001), 163.13% (p≤0.001), 92.42%, and 63.25% in G5, G6, G7, and G9 groups, respectively, than G4. However, the level of liver telomerase was reported to be increased by 59.09% (p≤0.001), 68.18% (p≤0.001), and 68.18% (p≤0.001) in the G6, G7, and G8, respectively as compared to the G4. Similarly, the telomerase level in the thigh muscle was significant increased by 15.79% in the G7 group as compared with the G4 group. Besides, the level of citrate synthase was significantly increased by 5.89% in the G8 group as compared to the G2 group. Altogether, the Biofield Treated/Blessed test formulation and Biofield Treatment per se to the animals could be an effective approach to improve the functioning of VDR (liver and adipose tissue), telomerase enzyme (liver and thigh muscle), and mitochondrial activity in terms of citrate synthase (substrate of kreb’s cycle). Therefore, the findings could be used against bone-related disorders (osteoporosis, fracture, scoliosis, and bone cancer), deficiency of vitamin D3, and mitochondrial dysfunction in associate with age-related and neurodegeneration disorders such as Alzheimer’s disease, dementias, brain cancer, epilepsy and Parkinson’s. The data showed a significant reduction of disease progression and all other disease-related complications/symptoms in the preventive treatment groups viz. G6, G7, G8, and G9.
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi and Snehasis Jana
Juniper Publishers
14-Jun-2021
English
Journal Article
An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds
Pharmaceuticals
<p style="text-align:justify;">The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness. The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis. The present study was aimed to evaluate the impact of biofield treatment on these properties of four pharmaceutical compounds such as urea, thiourea, sodium carbonate, and magnesium sulphate, using spectroscopic analysis. Each compound was divided into two groups, referred as control and treatment. The control groups remained as untreated and treatment group of each compound received Mr. Trivedi’s biofield treatment. Control and treated samples of each compound were characterized using Fourier-Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopy. FT-IR spectra of biofield treated urea showed the shifting of C=O stretching peak towards lower frequency (1684→1669 cm-1) and N-H stretching peak towards higher frequency (3428→3435 cm-1) with respect to control. A shift in frequency of C-N-H bending peak was also observed in treated sample as compared to control i.e. (1624→1647 cm-1). FT-IR spectra of thiourea showed upstream shifting of NH2 stretching peak (3363→3387 cm-1) as compared to control, which may be due to decrease in N-H bond length. Also, the change in frequency of N-C-S bending peak (621→660 cm-1) was observed in treated thiourea that could be due to some changes in bond angle after biofield treatment. Similarly, treated sample of sodium carbonate showed decrease in frequency of C-O bending peak (701→690 cm-1) and magnesium sulphate showed increase in frequency of S-O bending peak (621→647 cm-1) as compared to control, which indicated that bond angle might be altered after biofield treatment on respective samples. UV-Vis spectra of biofield treated urea showed shift in lambda max (λmax) towards higher wavelength (201→220 nm) as compared to control sample, whereas other compounds i.e. thiourea, sodium carbonate, and magnesium sulphate showed the similar λmax to their respective control. These findings conclude that biofield treatment has significant impact on spectral properties of tested pharmaceutical compounds which might be due to some changes happening at atomic level of compounds, and leading to affect the bonding and structural properties of compounds.</p>
Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Ragini Singh, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/an-impact-of-biofield-treatment-on-spectroscopic-characterization-of-pharmaceutical-compounds-2329-6798-1000159.php?aid=57988" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/an-impact-of-biofield-treatment-on-spectroscopic-characterization-of-pharmaceutical-compounds-2329-6798-1000159.php?aid=57988</a>
Omics Publishing Group
August 04, 2015
English
Journal Article
10.4172/2329-6798.1000159
An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential Using BACTEC 460/MGIT-TB System
Microbiology
<p style="text-align:justify;">The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as compared to control in extensive drug resistance (XDR) strains. Pyrazinamide showed 300% susceptibility as compared to control in multidrug resistance (MDR) strains. Isoniazide did not show any improvement of susceptibility pattern against treated either in XDR or MDR strains of Mycobacterium as compared to control. Besides susceptibility, the resistance pattern of treated group was reduced in case of isoniazide (26.7%), rifampicin (27.6%), pyrazinamide (31.4%), ethambutol (33.43%) and streptomycin (41.3%) as compared to the untreated group of XDR strains. The MIC values of few antimicrobials were also altered in the treated group of Mycobacterium smegmatis. There was a significant reduction observed in MIC values of linezolid (8.0 to 2.0 µg/ml) and tobramycin (2.0 to 1.0 µg/ml), however, very slight changes occurred in the remaining antimicrobials of treated samples. There was no change of MIC values in the strain of Mycobacterium tuberculosis after biofield treatment. Biofield treatment effect on Mycobacterium against anti-tubercular drugs might be due to altered ligand-receptor/protein interactions at either enzymatic and/or genetic level with respect to anti-mycobacterium susceptibility and MIC values of antimicrobials.</p>
Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, Snehasis Jana
<a href="https://www.omicsonline.org/open-access/an-impact-of-biofield-treatment-antimycobacterial-susceptibility-potential-using-bactec-460mgittb-system-2161-1068-1000189.php?aid=59486" target="_blank" rel="noreferrer noopener">https://www.omicsonline.org/open-access/an-impact-of-biofield-treatment-antimycobacterial-susceptibility-potential-using-bactec-460mgittb-system-2161-1068-1000189.php?aid=59486</a>
Omics Publishing Group
July 27, 2015
English
Journal Article
10.4172/2161-1068.1000189
An Impact of Energy of Consciousness (The Trivedi Effect®) on the Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate
Nutraceuticals
<p style="text-align:justify;">Magnesium gluconate is a potent antioxidant used for the prevention and treatment of hypomagnesia. The current research was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably changed from range -37.52% to 180.14% compared with the control sample. The average crystallite size was reduced in the treated sample by 0.11% compared with the control sample. PSD analysis revealed that the particle sizes in the treated sample at d10 and d50 values were significantly decreased by 11.36% and 8.25%, respectively, while the particle size at d90 was increased in the treated sample by 2.43% compared with the control sample. The treated sample’s surface area was significantly increased by 9.54% compared with the control sample. The FT-IR and UV-vis analysis showed that the structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed the four steps thermal degradation of the both samples and the total weight loss of the treated sample was reduced by 2.32% compared with the control sample. This result indicated that the thermal stability of the treated sample was improved compared with the control sample. The DSC analysis revealed that the melting temperature of the treated sample (171.05°C) was decreased by 0.17% compared with the control sample (171.34°C). The latent heat of fusion was decreased by 1.16% in the treated sample compared with the control sample. The current study infers that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging and other chronic infections.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=362&doi=10.11648/j.bs.20170302.11" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=362&doi=10.11648/j.bs.20170302.11</a>
Science Publishing Group
March 14, 2017
English
Journal Article
10.11648/j.bs.20170302.11
An Impact of the Trivedi Effect® - Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes
Nutraceuticals
<p style="text-align:justify;">Due to the increased popularity of herbomineral preparations in the healthcare sector, a new proprietary herbomineral formulation was formulated consisting of ashwagandha root extract and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells. The herbomineral formulation was divided into two parts, one defined as the control, while the other part was treated with the Biofield Energy Healing Treatment performed from a remote distance by twenty renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The splenocyte cells were exposed to test formulations at concentration from 0.00001053 to 10.53 µg/mL and were analyzed after 48 hours for cell viability using MTT assay. The expression of the cytokines (TNF-α, IFN-γ, IL-1β, and MIP-1α) was determined using ELISA assay. The cell viability data showed that all the tested concentration ranges were found to be safe with percentage cell viability at more than 80%. Further, TNF-α expression was significantly inhibited in the Biofield Treated test formulation group with respect to the vehicle control, while at 0.001053 and 0.1053 µg/mL, the expression was suppressed by 1.70% and 8.16%, respectively in the Biofield Treated test formulation compared to the untreated formulation. However, a significant immunosuppression was reported in IFN-γ expression at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The MIP-1α expression was inhibited by 8.31%, 21.53%, and 8.70% at 0.0001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated formulation compared with the untreated test formulation. However, IL-1β expression was significantly suppressed by 19.72% at concentration 0.00001053 µg/mL in the Biofield Treated test formulation compared with the untreated test formulation. Thus, the down-regulation of tested cytokines and chemokines in the Biofield Energy Healing test formulation might be applicable for controlling acute and chronic inflammation in many clinical diseases. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory activity of the test formulation, which can be useful against autoimmune disorders. Biofield Treated Test formulation may also be useful in anti-aging, anti-inflammatory, stress management and in preventing immune-mediated tissue damage in organ transplants by improving overall health and quality of life.</p>
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana
<a href="http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20160406.14" target="_blank" rel="noreferrer noopener">http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=118&doi=10.11648/j.ajls.20160406.14</a>
Science Publishing Group
December 08, 2016
English
Journal Article
10.11648/j.ajls.20160406.14